Ameliorative effect of vitamins (α-tocopherol and ascorbic acid) on PCB (Aroclor 1254) induced oxidative stress in rat epididymal sperm

• Published in: Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2007-02, Vol.23 (2), p.239-245
• Main Authors: Krishnamoorthy, G., Venkataraman, P., Arunkumar, A., Vignesh, R.C., Aruldhas, M.M., Arunakaran, J.
• Format: Article
• Language: English
• Subjects: alpha-Tocopherol - pharmacology; Animals; Antioxidants - pharmacology; Antithyroid Agents - toxicity; Ascorbic Acid - pharmacology; Biological and medical sciences; Chlorodiphenyl (54% Chlorine) - toxicity; Drug Therapy, Combination; Embryology: invertebrates and vertebrates. Teratology; Epididymis - drug effects; Fundamental and applied biological sciences. Psychology; Lipid Peroxidation - drug effects; Male; Medical sciences; Oxidative Stress - drug effects; Oxidoreductases - metabolism; Polychlorinated biphenyls; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Sperm; Sperm Motility - drug effects; Spermatozoa - drug effects; Spermatozoa - metabolism; Teratology. Teratogens; Toxicology; Vitamins
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/j.reprotox.2006.12.004

The aim of this study was to investigate the possible protective role of vitamins on PCB (Aroclor 1254)-induced spermiotoxicity using qualitative, quantitative and biochemical approaches. Adult male albino rats of Wistar strain were randomly divided into four groups, each group consists of six animals. The control group received corn oil, the second group of rats were administered Aroclor 1254 at a dose of 2 mg/kg bw/day intraperitoneally for 30 days. The third group of rats were treated with Aroclor 1254 along with α-tocopherol (50 mg/kg of bw/day) for 30 days, while the fourth group of rats were treated with Aroclor 1254 along with ascorbic acid (100 mg/kg bw/day) orally for 30 days. Twenty-four hours after the last treatment, control and experimental animals were killed by decapitation. Sperm was collected from the cauda epididymal region and its count and motility were detected. Sperm was sonicated and used for the estimation of reactive oxygen species (ROS) [hydroxyl radical (HO •) and hydrogen peroxide (H 2O 2)], non-enzymic antioxidants [α-tocopherol, ascorbic acid and reduced glutathione (GSH)], activity of enzymic antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) glutathione reductase (GR) and glutathione- S-transferase (GST)] and lipid peroxidation (LPO). The result of this experiment shows that PCB significantly decreases the level of α-tocopherol, ascorbic acid and GSH and the activities of SOD, CAT, GPx, GR and GST with elevated levels of ROS and LPO. In addition, decreased epididymal sperm motility and count were observed. Simultaneous supplementation with α-tocopherol and ascorbic acid restored these parameters to that of normal range. In conclusion, α-tocopherol and ascorbic acid exhibited protective effect on sperm by inhibiting PCB-induced ROS generation.