Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation

This study aims to evaluate, in vitro, the effect of Aloe vera associated with endodontic medication, with or without laser photobiomodulation (FTL) irradiation in FP6 human pulp fibroblasts. The materials were divided into eight groups: CTR - control; CL - FTL alone; AA - Aloe vera with distilled w...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2018-01, Vol.178, p.348-354
Main Authors: Carvalho, Nayane Chagas, Guedes, Simone Alves Garcez, Albuquerque-Júnior, Ricardo Luiz Cavalcanti, de Albuquerque, Diana Santana, de Souza Araújo, Adriano Antunes, Paranhos, Luiz Renato, Camargo, Samira Esteves Afonso, Ribeiro, Maria Amália Gonzaga
Format: Article
Language:English
Subjects:
Aloe
Aloe vera
Calcium
Calcium hydroxide
Cell survival
Cytotoxicity
Distilled water
Drugs
Endodontic medication
Fibroblasts
Flowers & plants
Genotoxicity
Genotype & phenotype
Hydroxyapatite
Irradiation
Laser photobiomodulation
Slaked lime
Toxicity
Variance analysis
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Summary:This study aims to evaluate, in vitro, the effect of Aloe vera associated with endodontic medication, with or without laser photobiomodulation (FTL) irradiation in FP6 human pulp fibroblasts. The materials were divided into eight groups: CTR - control; CL - FTL alone; AA - Aloe vera with distilled water; AL - Aloe vera with distilled water and FTL; HA - calcium hydroxide P.A. with distilled water; HL - calcium hydroxide P.A. with distilled water and FTL; HAA - calcium hydroxide P.A. with Aloe vera and distilled water; HAL - calcium hydroxide P.A. with Aloe vera, distilled water, and FTL. The cytotoxicity was evaluated by MTT assay at 24, 48, and 72h and the genotoxicity by micronucleus test assay. This study was performed in triplicate. Data obtained in both tests were statistically analyzed by ANOVA and Tukey's tests (p≤0.05). Group AA presented high genotoxicity and low cytotoxicity. After 24, 48, and 72h, the group HAA significantly reduced the cell viability. Interaction with FTL showed slightly increase cell viability after 24 and 48h in groups CL and HL (p
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2017.11.027