Small interfering RNA-mediated α-enolase knockdown suppresses glycolysis and proliferation of human glioma U251 cells in vitro

To investigate the role of α-enolase (ENO1) in regulating glucose metabolism and cell growth in human glioma cells. Glucose uptake and lactate generation were assessed to evaluate the changes in glucose metabolism in human glioma U251 cells with small interfering RNA (siRNA)-mediated ENO1 knockdown....

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Published in:Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2017-11, Vol.37 (11), p.1484
Main Authors: Luo, Qi-Sheng, Fu, Huang-de, Huang, Hai-Neng, Huang, Hua-Dong, Luo, Kun-Xiang, Li, Chuan-Yu, Qin, Cheng-Jian, Li, Xue-Yu, Luo, Hong-Cheng, Wang, Jun-Li, Tang, Qian-Li
Format: Article
Language:Chinese
Subjects:
Biomarkers, Tumor - genetics
Cell Line, Tumor
Cell Proliferation
DNA-Binding Proteins - genetics
Gene Knockdown Techniques
Glioma - pathology
Glycolysis
Humans
Phosphopyruvate Hydratase - genetics
RNA, Small Interfering - genetics
Transfection
Tumor Suppressor Proteins - genetics
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Summary:To investigate the role of α-enolase (ENO1) in regulating glucose metabolism and cell growth in human glioma cells. Glucose uptake and lactate generation were assessed to evaluate the changes in glucose metabolism in human glioma U251 cells with small interfering RNA (siRNA)-mediated ENO1 knockdown. MTT assay and 5-ethynyl-2'-deoxyuridine (EdU) staining were used to examine the cell growth and cell cycle changes following siRNA transfection of the cells. Transfection of U251 cells with siRNA-ENO1 markedly reduced glucose uptake (P=0.023) and lactate generation (P=0.007) in the cells and resulted in significant suppression of cell proliferation (*P
ISSN:1673-4254