Comparative transcriptomic analysis of larval and adult Malpighian tubules from the cotton bollworm Helicoverpa armigera

Malpighian tubules (MTs) are usually considered the key excretory and osmoregulatory organs of insects. However, increasing evidence has suggested that MTs perform many more functions than just osmoregulation. Until now, the molecular and physiological functions of MTs in the cotton bollworm (Helico...

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Bibliographic Details
Published in:Insect science 2018-12, Vol.25 (6), p.991-1005
Main Authors: Yuan, Yi‐Yang, Li, Mei, Fan, Fan, Qiu, Xing‐Hui
Format: Article
Language:English
Subjects:
Adults
Animals
Carbohydrate metabolism
Carbohydrates
Cotton
Detoxification
Diuresis
Diuresis - genetics
Ecological adaptation
Gene expression
Gene Expression Profiling
Gene Ontology
Helicoverpa armigera
Immunity
Insects
Larva - genetics
Larvae
Malpighian tubules
Malpighian Tubules - metabolism
Metabolism
Molecular Sequence Annotation
Moths - anatomy & histology
Moths - genetics
Moths - growth & development
Moths - physiology
Organs
Osmoregulation
Physiology
Proteins
Reproduction
Ribonucleic acid
RNA
Sexual dimorphism
Technology assessment
Transcription
transcriptome
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Summary:Malpighian tubules (MTs) are usually considered the key excretory and osmoregulatory organs of insects. However, increasing evidence has suggested that MTs perform many more functions than just osmoregulation. Until now, the molecular and physiological functions of MTs in the cotton bollworm (Helicoverpa armigera), a very important agricultural pest, are largely unknown. In this study, the transcriptomes of H. armigera MTs from larvae, male adults and female adults were sequenced using RNA‐Seq technology, and comparative analyses of transcriptomes between two life stages (larval and adult) and between adult sexes were conducted. We generated a total of 84 643 high‐quality unigenes, and identified a large number of abundant transcripts putatively encoding proteins involved in diuresis, detoxification, immunity, carbohydrate transport and metabolism, development and reproduction. We found that the expression pattern of unigenes was relatively similar between female and male adult MTs, but different between larval and adult MTs. Our data suggest that insect MTs may take multiple physiological functions as versatile organs. The extensive alterations in gene expression in MTs occurred from larvae to adults reflect an ecological adaptation to different feeding habits. Sexual dimorphism in the cotton bollworm is somewhat indicated by the transcriptional difference of genes related to carbohydrate metabolism, detoxification, immunity and reproduction in the MTs of male and female adults.
ISSN:1672-9609
1744-7917
DOI:10.1111/1744-7917.12561