Effect of Lactobacillus rhamnosus on the response of Galleria mellonella against Staphylococcus aureus and Escherichia coli infections

This study evaluated the prophylactic effects of the live or heat-killed probiotic strain Lactobacillus rhamnosus ATCC 7469 in Galleria mellonella , inoculated with Staphylococcus aureus or Escherichia coli. L. rhamnosus suspension was prepared and a part of it was autoclaved to obtain heat-killed l...

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Bibliographic Details
Published in:Archives of microbiology 2018-04, Vol.200 (3), p.383-389
Main Authors: Jorjão, Adeline Lacerda, de Oliveira, Felipe Eduardo, Leão, Mariella Vieira Pereira, Jorge, Antonio Olavo Cardoso, de Oliveira, Luciane Dias
Format: Article
Language:English
Subjects:
Autoclaving
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
E coli
Ecology
Escherichia coli
Galleria mellonella
Heat
Hemocytes
Hemolymph
Inoculation
Lactobacilli
Lactobacillus rhamnosus
Larvae
Life Sciences
Melanization
Microbial Ecology
Microbiology
Nitric oxide
Original Paper
Probiotics
Staphylococcus aureus
Survival
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Summary:This study evaluated the prophylactic effects of the live or heat-killed probiotic strain Lactobacillus rhamnosus ATCC 7469 in Galleria mellonella , inoculated with Staphylococcus aureus or Escherichia coli. L. rhamnosus suspension was prepared and a part of it was autoclaved to obtain heat-killed lactobacilli. The larvae were inoculated of these suspensions and pathogenic. The survival of the larvae was observed during 7 days and after 24 h of inoculation haemocytes counted, melanization and nitric oxide production were analyzed. Larvae survival rate increased in the group inoculated with heat-killed L. rhamnosus , however, with no statistical difference. There was a significant increase in total haemocyte counts in all test groups. Haemolymph melanization and nitric oxide production were higher in the group inoculated with L. rhamnosus and infected with S. aureus . It was concluded that, in this model of infection, heat-killed L. rhamnosus ATCC 7469 promoted greater protection in Galleria mellonella infected with S. aureus or E. coli .
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-017-1441-7