Algoriphagus formosus sp. nov., isolated from coastal sediment

• Published in: Antonie van Leeuwenhoek 2018-06, Vol.111 (6), p.913-920
• Main Authors: Ye, Meng-Qi, Wang, Xu-Ting, Zhang, Jing, Chen, Guan-Jun, Du, Zong-Jun
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial Typing Techniques; Bacteroidetes - genetics; Base Composition - genetics; Biochemical tests; Biomedical and Life Sciences; Coastal plains; Deoxyribonucleic acid; DNA; Fatty acids; Genomes; Geologic Sediments - microbiology; Life Sciences; Lipids; Marine biology; Marine sediments; Medical Microbiology; Menaquinones; Microbiology; New species; Original Paper; pH effects; Phosphatidylethanolamine; Phospholipids; Phylogeny; Pigments; Plant Sciences; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seawater - microbiology; Sediment samplers; Sediments; Sodium chloride; Soil Science & Conservation
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-017-0990-8

A Gram-stain negative, facultative anaerobic, non-motile, strongly orange-pigmented and rod-shaped bacterium, designated XAY3209 T , was isolated from a marine sediment sample collected from the coast of Weihai, China. Strain XAY3209 T was found to grow optimally at 30 °C, at pH 7.0 and in the presence of 2.0% (w/v) NaCl. Its genomic DNA G+C content was 41.9 mol%. On the basis of 16S rRNA gene sequence similarity, the novel isolate belongs to the family Cyclobacteriaceae and is related to the genus Algoriphagus . It shares 98.1% 16S rRNA sequence identity with Algoriphagus marincola , its close phylogenetic relative, but did not show similarities more than 97% with other members of the genus Algoriphagus with validly published names. It contained menaquinone-7 (MK-7) as the sole respiratory quinone, iso-C 15:0 , iso-C 17:1 ω 9 c and Summed feature 3 (C 16:1 ω 7 c and/or iso-C 15:0 2-OH) as the major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid and five unidentified lipids. Results of physiological experiments, biochemical tests and genome average nucleotide identity value (with A. marincola MCCC 1F01203 T ) indicate that strain XAY3209 T is genetically and phenotypically distinct from the species of the genus Algoriphagus with validly published names. Strain XAY3209 T therefore represents a novel species, for which the name Algoriphagus formosus sp. nov. is proposed. The type strain is XAY3209 T (= KCTC 52842 T  = MCCC 1H00189 T ).