Electrode-based AC electrokinetics of proteins: A mini-review

Employing electric phenomena for the spatial manipulation of bioparticles from whole cells down to dissolved molecules has become a useful tool in biotechnology and analytics. AC electrokinetic effects like dielectrophoresis and AC electroosmosis are increasingly used to concentrate, separate and im...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2018-04, Vol.120, p.76-82
Main Authors: Laux, Eva-Maria, Bier, Frank F., Hölzel, Ralph
Format: Article
Language:English
Subjects:
AC electrokinetics
Analytical chemistry
Animals
Biotechnology
Deoxyribonucleic acid
Dielectrophoresis
DNA
Electricity
Electrodes
Electrokinetic effects
Electrokinetics
Electroosmosis
Electroosmosis - instrumentation
Electroosmosis - methods
Electrophoresis - instrumentation
Electrophoresis - methods
Equipment Design
Humans
Immobilization
Kinetics
Molecular chains
Nanofabrication
Osmosis
Photolithography
Proteins
Proteins - analysis
Quadrupoles
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Summary:Employing electric phenomena for the spatial manipulation of bioparticles from whole cells down to dissolved molecules has become a useful tool in biotechnology and analytics. AC electrokinetic effects like dielectrophoresis and AC electroosmosis are increasingly used to concentrate, separate and immobilize DNA and proteins. With the advance of photolithographical micro- and nanofabrication methods, novel or improved bioanalytical applications benefit from concentrating analytes, signal enhancement and locally controlled immobilization by AC electrokinetic effects. In this review of AC electrokinetics of proteins, the respective studies are classified according to their different electrode geometries: individual electrode pairs, interdigitated electrodes, quadrupole electrodes, and 3D configurations of electrode arrays. Known advantages and disadvantages of each layout are discussed. •Setups and applications of electrode-based protein AC electrokinetics are reviewed.•Coplanar electrode structures require higher frequencies than 3D electrode geometries.•AC electrokinetic effects depend more on electrode architecture than on the sample.•Sharp curvatures of electrodes are decisive for high electric field gradients.
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2017.11.010