Submerged and solid-state production of laccase and Mn-peroxidase by Panus tigrinus on olive mill wastewater-based media

• Published in: Journal of biotechnology 2003-01, Vol.100 (1), p.77-85
• Main Authors: Fenice, Massimiliano, Giovannozzi Sermanni, Giovanni, Federici, Federico, D'Annibale, Alessandro
• Format: Article
• Language: English
• Subjects: Basidiomycota - enzymology; Basidiomycota - growth & development; Biodegradation, Environmental; Biological and medical sciences; Bioreactors; Biotechnology; Cell Culture Techniques - methods; Cells, Cultured; Enzyme engineering; Fermentation; Fundamental and applied biological sciences. Psychology; Industrial Waste - prevention & control; Laccase; Methods. Procedures. Technologies; Mn-peroxidase; Olea; Olive mill wastewater; Olive Oil; Oxidoreductases - biosynthesis; Panus tigrinus; Peroxidases - biosynthesis; Phenols - metabolism; Plant Oils; Production of selected enzymes; Solutions; Waste Disposal, Fluid - methods; Water Pollutants, Chemical - metabolism
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/S0168-1656(02)00241-9

The possible use of olive-mill wastewater (OMW) as a growth medium for the production of extracellular laccase and manganese peroxidase (MnP) from the white-rot fungus Panus tigrinus ( P. tigrinus) CBS 577.79 was studied using a properly formulated OMW-based medium (2-fold diluted OMW supplemented with 0.5% sucrose and 0.1% yeast extract) either in a stirred-tank or an air-lift reactor. Solid-state fermentation (SSF) was also performed in a rotary drum reactor using maize stalks moistened with the OMW-based medium. Highest levels of laccase and manganese peroxidase activity were obtained in the stirred-tank reactor (4600±98 U l −1 on day 13) and in the air-lift reactor (410±22 on day 7), respectively. Based on total enzyme activities, SSF appears to be more suitable than LSF but the latter exhibits better volumetric productivities.