Prior exposure to restraint stress enhances 7,12-dimethylbenz( a)anthracene (DMBA) induced DNA damage in rats

Over the years, several lines of evidence have emerged supporting the role of stress in the development and progression of cancer. Stress can cause an increase in the production of reactive oxygen species (ROS) and decrease in the in vivo antioxidant defense systems. A ROS-induced DNA damage in peri...

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Bibliographic Details
Published in:FEBS letters 2006-07, Vol.580 (16), p.3995-3999
Main Authors: Muqbil, Irfana, Azmi, Asfar S., Banu, Naheed
Format: Article
Language:English
Subjects:
9,10-Dimethyl-1,2-benzanthracene - pharmacology
Animals
Comet Assay
DMBA
DNA damage
DNA Damage - drug effects
Dose-Response Relationship, Drug
Hepatocytes - drug effects
Hepatocytes - metabolism
Liver - metabolism
MDA
Oxidative Stress
Rats
reactive oxygen species
Restraint stress
Restraint, Physical
ROS
Single cell gel electrophoresis
Skin - cytology
Skin - drug effects
TBARS
thiobarbituric acid reactive species
Thiobarbituric Acid Reactive Substances - metabolism
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Summary:Over the years, several lines of evidence have emerged supporting the role of stress in the development and progression of cancer. Stress can cause an increase in the production of reactive oxygen species (ROS) and decrease in the in vivo antioxidant defense systems. A ROS-induced DNA damage in peripheral lymphocytes, liver and skin cells may be revealed by Comet assay. To test whether DNA is damaged by stress/DMBA/stress and DMBA, rats were exposed to multiple doses of DMBA in the presence and absence of restraint stress, and DNA damage was evaluated. Insignificant differences were detected in all the three cells tested (peripheral lymphocytes, liver and skin cells) between control and stress treatment in terms of frequencies of damaged DNA. The extent of DNA migration was enhanced in DMBA treated rats in a dose dependent manner. Pre-stress DMBA treatment showed still higher frequencies of damage in comparison with control, stress alone or DMBA alone groups. Thus, prior exposure to stress clearly enhanced the DMBA induced DNA damage, especially so in the skin cells (target organ of the carcinogen application) than liver and peripheral lymphocytes as observed on the basis of the extent of DNA migration (tail DNA) during single cell gel electrophoresis.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2006.06.030