Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement

• Published in: Journal of agricultural and food chemistry 2017-12, Vol.65 (50), p.11029-11035
• Main Authors: You, Seung Kyou, Joo, Young-Chul, Kang, Dae Hee, Shin, Sang Kyu, Hyeon, Jeong Eun, Woo, Han Min, Um, Youngsoon, Park, Chulhwan, Han, Sung Ok
• Format: Article
• Language: English
• Subjects: Acetyl-CoA Carboxylase - genetics; Acetyl-CoA Carboxylase - metabolism; Animal Feed - analysis; Animals; Cattle - growth & development; Cattle - metabolism; Dietary Supplements - analysis; Fatty Acids - biosynthesis; Metabolic Engineering; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.7b04485

Saccharomyces cerevisiae is used for edible purposes, such as human food or as an animal feed supplement. Fatty acids are also beneficial as feed supplements, but S. cerevisiae produces small amounts of fatty acids. In this study, we enhanced fatty acid production of S. cerevisiae by overexpressing acetyl-CoA carboxylase, thioesterase, and malic enzyme associated with fatty acid metabolism. The enhanced strain pAMT showed 2.4-fold higher fatty acids than the wild-type strain. To further increase the fatty acids, various nitrogen sources were analyzed and calcium nitrate was selected as an optimal nitrogen source for fatty acid production. By concentration optimization, 672 mg/L of fatty acids was produced, which was 4.7-fold higher than wild-type strain. These results complement the low level fatty acid production and make it possible to obtain the benefits of fatty acids as an animal feed supplement while, simultaneously, maintaining the advantages of S. cerevisiae.