Effects of chitosan on oxidative stress and metallothioneins in aquatic worm Tubifex tubifex (Oligochaeta, Tubificidae)

• Published in: Chemosphere (Oxford) 2007-02, Vol.67 (1), p.167-175
• Main Authors: Mosleh, Yahia Y., Paris-Palacios, Séverine, Ahmed, Mohamed T., Mahmoud, F.M., Osman, M.A., Biagianti-Risbourg, Sylvie
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Animals; Annelida and closely related phyla: sipuncula. Echiura. Nemertinea; Applied ecology; Biological and medical sciences; Catalase - metabolism; Chito-gel; Chitosan - chemistry; Chitosan - toxicity; Ecotoxicology, biological effects of pollution; Fundamental and applied biological sciences. Psychology; General aspects; Glutathione Reductase - metabolism; Glutathione Transferase - metabolism; Invertebrates; Metallothionein - metabolism; Metallothioneins; Molecular Structure; Oligochaeta; Oligochaeta - drug effects; Oligochaeta - growth & development; Oligochaeta - metabolism; Oxidative stress; Oxidative Stress - drug effects; Tubifex; Tubifex tubifex; Tubificidae; Water Pollution - analysis
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2006.09.019

Chitosan is a natural polymer which has the property to elicit the natural defenses mechanism in plant and which can be an interesting biopesticides. It is then necessary to investigate the potential toxicity of chitosan for aquatic animal health. Metallothioneins (MTs) are low molecular weight proteins, mainly implicated in metal ion detoxification. Increase in MTs contents had been considered as a specific biomarker of metal exposure. However recently it has been demonstrated that MTs participate in several cellular functions such as regulation of growth and anti-oxidative defenses. Therefore, the induction of MTs has been investigated in the aquatic worms Tubifex tubifex exposed to chitosan. MTs levels in exposed worm increased significantly ( p > 0.05) after 2, 4, and 7 days of exposure to different concentrations of chitosan (maximum + 158.19 ± 10.2% after 2 days of exposure to 125 mg l −1 of chitosan). Several antioxidant parameters including glutathione (GSH), glutathione- S-transferase (GST), glutathione reducatse (GR), and catalase (CAT) were quantified in T. tubifex after 2, 4, and 7 days of exposure to chitosan. Exposure to chitosan had a negative effect on T. tubifex growth (maximum effect −6.11 ± 1.6% after 7 days with 125 mg l −1) demonstrating the toxic effect of the pesticide. This growth rate decrease was accompanied by a reduction in protein contents. The activity of catalase (CAT), glutathione- S-transferase (GST), and glutathione reductase (GR) increased in response to the chitosan demonstrating an oxidative stress in the worms.