Regeneration of Melia volkensii Guerke (Meliaceae) through direct somatic embryogenesis

Experiments were conducted to study plant regeneration through direct somatic embryogenesis using mature zygotic embryo and cotyledonary explants from seeds of Melia volkensii stored for 12 months. Explants were cultured on Murashige and Skoog (MS) medium supplemented with BAP, NAA and 2,4-D (0.5, 1...

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Bibliographic Details
Published in:New forests 2007-07, Vol.34 (1), p.73-81
Main Authors: Indieka, SA, Odee, D W, Muluvi, G M, Rao, K N, Machuka, J
Format: Article
Language:English
Subjects:
Melia
Meliaceae
Online Access:Get full text
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Summary:Experiments were conducted to study plant regeneration through direct somatic embryogenesis using mature zygotic embryo and cotyledonary explants from seeds of Melia volkensii stored for 12 months. Explants were cultured on Murashige and Skoog (MS) medium supplemented with BAP, NAA and 2,4-D (0.5, 1.0 and 2.0 mg l super(-1)) alone, and BAP (0.5, 1.0, 2.0 and 4.0 mg l super(-1)) in combination with 2,4-D or NAA (0.2 and 0.5 mg l super(-1)). After 4 weeks in culture, up to 60% of cotyledonary explants from the seeds stored for 12 months produced somatic embryos. Mature zygotic embryos failed to produce any somatic embryos. Subcultures of somatic embryos from cotyledonary explants of seeds stored for
ISSN:0169-4286
DOI:10.1007/s11056-007-9038-7