Cloning of a Novel Gene Encoding b-1,3-Xylosidase from a Marine Bacterium, Vibrio sp. Strain XY-214, and Characterization of the Gene Product

The b-1,3-xylosidase gene (xloA) of Vibrio sp. strain XY-214 was cloned and expressed in Escherichia coli. The xloA gene consisted of a 1,608-bp nucleotide sequence encoding a protein of 535 amino acids with a predicted molecular weight of 60,835. The recombinant b-1,3-xylosidase hydrolyzed b-1,3-xy...

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Bibliographic Details
Published in:Applied and environmental microbiology 2008-01, Vol.74 (1), p.305-308
Main Authors: Umemoto, Yoshiaki, Onishi, Ryosuke, Araki, Toshiyoshi
Format: Article
Language:English
Subjects:
Marine
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Summary:The b-1,3-xylosidase gene (xloA) of Vibrio sp. strain XY-214 was cloned and expressed in Escherichia coli. The xloA gene consisted of a 1,608-bp nucleotide sequence encoding a protein of 535 amino acids with a predicted molecular weight of 60,835. The recombinant b-1,3-xylosidase hydrolyzed b-1,3-xylooligosaccharides to D-xylose as a final product.
ISSN:0099-2240
1098-5336