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Effect of low density lipoprotein on DNA integrity of freezing–thawing boar sperm by neutral comet assay
A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing–thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (...
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| Published in: | Animal reproduction science 2007-06, Vol.99 (3), p.401-407 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c496t-2486b8559b1aeb0dbd468bd621eaac8f79a1128a5435667740d4f160ec227ff3 |
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| cites | cdi_FETCH-LOGICAL-c496t-2486b8559b1aeb0dbd468bd621eaac8f79a1128a5435667740d4f160ec227ff3 |
| container_end_page | 407 |
| container_issue | 3 |
| container_start_page | 401 |
| container_title | Animal reproduction science |
| container_volume | 99 |
| creator | Jiang, Zhong-Liang Li, Qing-Wang Li, Wen-Ye Hu, Jian-Hong Zhao, Hong-Wei Zhang, Shu-Shan |
| description | A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing–thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (
p
<
0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25
ml than in 0.5
ml types of straw (
p
<
0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (
p
>
0.05). |
| doi_str_mv | 10.1016/j.anireprosci.2006.08.022 |
| format | article |
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p
<
0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25
ml than in 0.5
ml types of straw (
p
<
0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (
p
>
0.05).</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2006.08.022</identifier><identifier>PMID: 17045432</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>additives ; animal breeding ; Animals ; assays ; Boar ; boars ; cold injury ; Comet Assay - veterinary ; cryopreservation ; Cryopreservation - veterinary ; Cryoprotective Agents - pharmacology ; DNA damage ; DNA Damage - drug effects ; DNA integrity ; freeze-thaw cycles ; freezing ; germplasm conservation ; Lipoproteins, LDL - pharmacology ; low density lipoprotein ; Low density lipoprotein (LDL) ; Male ; Neutral comet assay ; optimization ; phase transition ; protective effect ; Sperm ; spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - physiology ; Swine - physiology ; temporal variation</subject><ispartof>Animal reproduction science, 2007-06, Vol.99 (3), p.401-407</ispartof><rights>2006 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496t-2486b8559b1aeb0dbd468bd621eaac8f79a1128a5435667740d4f160ec227ff3</citedby><cites>FETCH-LOGICAL-c496t-2486b8559b1aeb0dbd468bd621eaac8f79a1128a5435667740d4f160ec227ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17045432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Zhong-Liang</creatorcontrib><creatorcontrib>Li, Qing-Wang</creatorcontrib><creatorcontrib>Li, Wen-Ye</creatorcontrib><creatorcontrib>Hu, Jian-Hong</creatorcontrib><creatorcontrib>Zhao, Hong-Wei</creatorcontrib><creatorcontrib>Zhang, Shu-Shan</creatorcontrib><title>Effect of low density lipoprotein on DNA integrity of freezing–thawing boar sperm by neutral comet assay</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing–thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (
p
<
0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25
ml than in 0.5
ml types of straw (
p
<
0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (
p
>
0.05).</description><subject>additives</subject><subject>animal breeding</subject><subject>Animals</subject><subject>assays</subject><subject>Boar</subject><subject>boars</subject><subject>cold injury</subject><subject>Comet Assay - veterinary</subject><subject>cryopreservation</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>DNA integrity</subject><subject>freeze-thaw cycles</subject><subject>freezing</subject><subject>germplasm conservation</subject><subject>Lipoproteins, LDL - pharmacology</subject><subject>low density lipoprotein</subject><subject>Low density lipoprotein (LDL)</subject><subject>Male</subject><subject>Neutral comet assay</subject><subject>optimization</subject><subject>phase transition</subject><subject>protective effect</subject><subject>Sperm</subject><subject>spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>Swine - physiology</subject><subject>temporal variation</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNkc-OFCEQh4nRuOPqKyhevHVb0D1AHzfj-ifZ6MH1TGi6WJl0wwiMm_HkO_iGPolMZhI9eoKkvqr65StCXjJoGTDxetua4BPuUszWtxxAtKBa4PwBWTElu4bzjj8kK-ikavqOwwV5kvMWAKQQw2NywST061pYke21c2gLjY7O8Z5OGLIvBzr7XazjC_pAY6BvPl5RHwrepWOxsi4h_vDh7vfPX-Wrua8_OkaTaN5hWuh4oAH3JZmZ2rhgoSZnc3hKHjkzZ3x2fi_J7dvr28375ubTuw-bq5vG9oMoDe-VGNV6PYzM4AjTOPVCjZPgDI2xysnBMMaVqfnXQkjZw9Q7JgAt59K57pK8Oo2t-b_tMRe9-Gxxnk3AuM-aDZIrCaqCwwm01WNO6PQu-cWkg2agj571Vv_jWR89a1C6eq69z89L9uOC09_Os9gKvDgBzkRtqresv3zmwLp6g3oh1VVicyKwuvjuMem6BIPFqa60RU_R_0eQP6TZoIs</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Jiang, Zhong-Liang</creator><creator>Li, Qing-Wang</creator><creator>Li, Wen-Ye</creator><creator>Hu, Jian-Hong</creator><creator>Zhao, Hong-Wei</creator><creator>Zhang, Shu-Shan</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20070601</creationdate><title>Effect of low density lipoprotein on DNA integrity of freezing–thawing boar sperm by neutral comet assay</title><author>Jiang, Zhong-Liang ; Li, Qing-Wang ; Li, Wen-Ye ; Hu, Jian-Hong ; Zhao, Hong-Wei ; Zhang, Shu-Shan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-2486b8559b1aeb0dbd468bd621eaac8f79a1128a5435667740d4f160ec227ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>additives</topic><topic>animal breeding</topic><topic>Animals</topic><topic>assays</topic><topic>Boar</topic><topic>boars</topic><topic>cold injury</topic><topic>Comet Assay - veterinary</topic><topic>cryopreservation</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>DNA damage</topic><topic>DNA Damage - drug effects</topic><topic>DNA integrity</topic><topic>freeze-thaw cycles</topic><topic>freezing</topic><topic>germplasm conservation</topic><topic>Lipoproteins, LDL - pharmacology</topic><topic>low density lipoprotein</topic><topic>Low density lipoprotein (LDL)</topic><topic>Male</topic><topic>Neutral comet assay</topic><topic>optimization</topic><topic>phase transition</topic><topic>protective effect</topic><topic>Sperm</topic><topic>spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>Swine - physiology</topic><topic>temporal variation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Zhong-Liang</creatorcontrib><creatorcontrib>Li, Qing-Wang</creatorcontrib><creatorcontrib>Li, Wen-Ye</creatorcontrib><creatorcontrib>Hu, Jian-Hong</creatorcontrib><creatorcontrib>Zhao, Hong-Wei</creatorcontrib><creatorcontrib>Zhang, Shu-Shan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Zhong-Liang</au><au>Li, Qing-Wang</au><au>Li, Wen-Ye</au><au>Hu, Jian-Hong</au><au>Zhao, Hong-Wei</au><au>Zhang, Shu-Shan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of low density lipoprotein on DNA integrity of freezing–thawing boar sperm by neutral comet assay</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>99</volume><issue>3</issue><spage>401</spage><epage>407</epage><pages>401-407</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing–thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (
p
<
0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25
ml than in 0.5
ml types of straw (
p
<
0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (
p
>
0.05).</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>17045432</pmid><doi>10.1016/j.anireprosci.2006.08.022</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-4320 |
| ispartof | Animal reproduction science, 2007-06, Vol.99 (3), p.401-407 |
| issn | 0378-4320 1873-2232 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19728708 |
| source | ScienceDirect Journals |
| subjects | additives animal breeding Animals assays Boar boars cold injury Comet Assay - veterinary cryopreservation Cryopreservation - veterinary Cryoprotective Agents - pharmacology DNA damage DNA Damage - drug effects DNA integrity freeze-thaw cycles freezing germplasm conservation Lipoproteins, LDL - pharmacology low density lipoprotein Low density lipoprotein (LDL) Male Neutral comet assay optimization phase transition protective effect Sperm spermatozoa Spermatozoa - drug effects Spermatozoa - physiology Swine - physiology temporal variation |
| title | Effect of low density lipoprotein on DNA integrity of freezing–thawing boar sperm by neutral comet assay |
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