Adherence-Inhibitory Intestinal Immunoglobulin A Antibody Response in Baboons Elicited by Use of a Synthetic Intranasal Lectin-Based Amebiasis Subunit Vaccine

We designed an amebiasis subunit vaccine that is constructed by using four peptide epitopes of the galactose-inhibitable lectin heavy subunit that were recognized by intestinal secretory immunoglobulin A (IgA) antibodies from immune human subjects. These epitopes are contained in the region encompas...

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Bibliographic Details
Published in:Infection and Immunity 2007-08, Vol.75 (8), p.3812-3822
Main Authors: Abd Alla, Mohamed D, White, Gary L, Rogers, Tyson B, Cary, Max E, Carey, David W, Ravdin, Jonathan I
Format: Article
Language:English
Subjects:
Administration, Intranasal
Amibiasis
Animals
Antibodies, Protozoan - immunology
Applied microbiology
Biological and medical sciences
Blood - immunology
Blotting, Western
Cell Adhesion - immunology
CHO Cells
Cholera Toxin - immunology
Cricetinae
Cricetulus
Entamoeba histolytica
Entamoebiasis - immunology
Entamoebiasis - prevention & control
Enzyme-Linked Immunosorbent Assay
Epitopes - genetics
Epitopes - immunology
Female
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Fungal and Parasitic Infections
Human protozoal diseases
Humans
Immunoglobulin A, Secretory - immunology
Infectious diseases
Intestines - immunology
Lectins - genetics
Lectins - immunology
Medical sciences
Microbiology
Papio
Papio anubis
Parasitic diseases
Protozoal diseases
Protozoan Proteins - genetics
Protozoan Proteins - immunology
Protozoan Vaccines - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Subunit - immunology
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Summary:We designed an amebiasis subunit vaccine that is constructed by using four peptide epitopes of the galactose-inhibitable lectin heavy subunit that were recognized by intestinal secretory immunoglobulin A (IgA) antibodies from immune human subjects. These epitopes are contained in the region encompassing amino acids 758 to 1134 of the lectin heavy subunit, designated LC3. Baboons (Papio anubis) are natural hosts for Entamoeba histolytica; naturally infected baboons raised in captivity possess serum IgA antibodies to the same four LC3 epitopes as humans. Uninfected, seronegative baboons received four intranasal immunizations at 7-day intervals with the synthetic peptide vaccine (400, 800, or 1,600 μg per nostril) with cholera toxin (20 μg) as the adjuvant. As determined by an enzyme-linked immunosorbent assay (ELISA), each dose of the peptide vaccine elicited antipeptide serum IgA and IgG and intestinal IgA antibody responses in all six immunized baboons by day 28, 7 days after the last immunization (P,
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.00341-07