Identification of unamplified genomic DNA sequences using gold nanoparticle probes and a novel thin film photodetector

This paper describes a novel colorimetric method for detection of nucleic acid targets in a homogeneous format with improved sensitivity by means of a system based on the combination of a tunable monochromatic light source and an amorphous/nanocrystalline silicon photodetector that detects color and...

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Bibliographic Details
Published in:Journal of non-crystalline solids 2008-05, Vol.354 (19-25), p.2580-2584
Main Authors: Martins, R., Baptista, P., Silva, L., Raniero, L., Doria, G., Franco, R., Fortunato, E.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Applied sciences
Biological and medical sciences
Biosensors
Cross-disciplinary physics: materials science
rheology
Dna, deoxyribonucleoproteins
Electronics
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Materials science
Mycobacterium tuberculosis
Nanocrystalline materials
Nanoparticles
Nanopowders
Nanoscale materials and structures: fabrication and characterization
Nucleic acids
Optoelectronic devices
Physics
Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices
Sensors
Silicon
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Summary:This paper describes a novel colorimetric method for detection of nucleic acid targets in a homogeneous format with improved sensitivity by means of a system based on the combination of a tunable monochromatic light source and an amorphous/nanocrystalline silicon photodetector that detects color and light intensity changes undergone by samples/assays containing tailored gold nanoparticles probes. This new low cost, portable, fast and simple optoelectronic platform, with the possibility to be re-used, permits detection of at least 400 fentomole of specific DNA sequences without target or signal amplification and was applied to the rapid detection of human pathogens in large variety of clinical samples such as Mycobacterium tuberculosis.
ISSN:0022-3093
1873-4812
DOI:10.1016/j.jnoncrysol.2007.09.074