Stimulation of interleukin-13 expression by human T-cell leukemia virus type 1 oncoprotein Tax via a dually active promoter element responsive to NF-{kappa}B and NFAT

• Published in: Journal of general virology 2008-11, Vol.89 (11), p.2788-2798
• Main Authors: Silbermann, Katrin, Schneider, Grit, Grassmann, Ralph
• Format: Article
• Language: English
• Subjects: Human T-lymphotropic virus 1
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/vir.0.2008/003699-0

Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany Correspondence Katrin Silbermann Katrin.Silbermann{at}viro.med.uni-erlangen.de The human T-cell leukemia virus type 1 (HTLV-1) Tax oncoprotein transforms human lymphocytes and is critical for the pathogenesis of HTLV-1-induced adult T-cell leukaemia. In HTLV-transformed cells, Tax upregulates interleukin (IL)-13, a cytokine with proliferative and anti-apoptotic functions that is linked to leukaemogenesis. Tax-stimulated IL-13 is thought to result in autocrine stimulation of HTLV-infected cells and thus may be relevant to their growth. The causal transactivation of the IL-13 promoter by Tax is predominantly dependent on a nuclear factor of activated T cells (NFAT)-binding P element. Here, it was shown that the isolated IL-13 Tax-responsive element (IL13TaxRE) was sufficient to mediate IL-13 transactivation by Tax and NFAT1. However, cyclosporin A, a specific NFAT inhibitor, revealed that Tax transactivation of IL13TaxRE or wild-type IL-13 promoter was independent of NFAT and that NFAT did not contribute to IL-13 upregulation in HTLV-transformed cells. By contrast, Tax stimulation was repressible by an efficient nuclear factor (NF)- B inhibitor (IkBaDN), indicating the requirement for NF- B. The capacity of NF- B to stimulate IL13TaxRE was demonstrated by a strong response to NF- B in reporter assays and by direct binding of NF- B to IL13TaxRE. Thus, IL13TaxRE in the IL-13 promoter represents a dually active promoter element responsive to NF- B and NFAT. Together, these results indicate that Tax causes IL-13 upregulation in HTLV-1-infected cells via NF- B. The HTLV group from Erlangen dedicates this manuscript to the memory of Professor Ralph Grassmann.