Regulation of the Xenopus Xsox17a sub(1) promoter by co-operating VegT and Sox17 sites

The gene encoding the Sox F-group transcription factor Xsox17a sub(1) is specifically expressed throughout the entire region of the Xenopus blastula fated to become endoderm, and is important in controlling endodermal development. Xsox17a sub(1) is a direct target of the maternal endodermal determin...

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Bibliographic Details
Published in:Developmental biology 2007-10, Vol.310 (2), p.402-415
Main Authors: Howard, Laura, Rex, Maria, Clements, Debbie, Woodland, Hugh R
Format: Article
Language:English
Subjects:
Freshwater
Xenopus laevis
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Summary:The gene encoding the Sox F-group transcription factor Xsox17a sub(1) is specifically expressed throughout the entire region of the Xenopus blastula fated to become endoderm, and is important in controlling endodermal development. Xsox17a sub(1) is a direct target of the maternal endodermal determinant VegT and of Sox17 itself. We have analysed the promoter of the Xenopus laevis Xsox17a sub(1) gene by transgenesis, and have identified two important control elements which reside about 9 kb upstream at the start of transcription. These elements individually drive transgenic endodermal expression in the blastula and gastrula. One contains functional, cooperating VegT and Sox-binding consensus sites. The Sox sites in this region are occupied in vivo. The other responds to TGF-b signals like Activin or Nodals that act through Smad2/3. We propose that these two regions co-operate in regulating the early endodermal expression of the Xsox17a sub(1) gene.
ISSN:0012-1606
1095-564X
DOI:10.1016/j.ydbio.2007.07.028