In vitro invasion capacity of Salmonella Typhimurium DT9 isolates sourced from humans and layer hen environments

Summary In Australia, Salmonella Typhimurium definitive type 9 is frequently isolated during foodborne outbreaks of salmonellosis. Multiple‐locus variable number tandem repeat analysis (MLVA) trace back investigations frequently identify isolate distribution patterns that may be epidemiologically li...

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Published in:Zoonoses and public health 2018-02, Vol.65 (1), p.e259-e264
Main Authors: McWhorter, A. R., Phan, G., Hocking, H., Chousalkar, K. K.
Format: Article
Language:English
Subjects:
Bacteria
Caco2 cells
Capacity
Cells
Colon
Colorectal cancer
Disease outbreaks
Epidemiology
Fingerprinting
Foodborne diseases
invasion
Microbiological strains
MLVA
Multiplicity of infection
Neoplasms
Outbreaks
Recovery
repetitive element PCR
Salmonella
Salmonella Typhimurium
Salmonellosis
Stationary phase
Virulence
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Phan, G.
Hocking, H.
Chousalkar, K. K.
description Summary In Australia, Salmonella Typhimurium definitive type 9 is frequently isolated during foodborne outbreaks of salmonellosis. Multiple‐locus variable number tandem repeat analysis (MLVA) trace back investigations frequently identify isolate distribution patterns that may be epidemiologically linked to disease outbreaks. In this study, the in vitro virulence potential of S. Typhimurium DT9 isolates possessing different MLVA patterns (03 15 07 11 550, 03 24 11 10 523, 03 15 08 11 550 and 03 14 08 11 550) isolated from either humans or layer hens was assessed using a human colon carcinoma cell line. Four strains per MLVA from each host for a total of 32 isolates were included in these experiments. Bacteria were grown to stationary phase and added to cells at a multiplicity of infection of 100. Across all isolates, mean percent recovery ranged from 7.1 ± 1.1 to 33.3 ± 7.1%. The layer hen isolate, KC900 (MLVA profile 03 15 08 11 550), exhibited the greatest invasion with a mean percent recovery of 33.3 ± 7.1%. Overall, layer hen isolates of S. Typhimurium DT9 had significantly higher invasion into Caco2 cells than human isolates (p = .0021). RAPD and enterobacterial repetitive intergenic consensus genomic fingerprinting was also performed. Irrespective of source, the SalmonellaDT9 isolates included in this study exhibited similar fingerprint patterns.
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R. ; Phan, G. ; Hocking, H. ; Chousalkar, K. K.</creator><creatorcontrib>McWhorter, A. R. ; Phan, G. ; Hocking, H. ; Chousalkar, K. K.</creatorcontrib><description>Summary In Australia, Salmonella Typhimurium definitive type 9 is frequently isolated during foodborne outbreaks of salmonellosis. Multiple‐locus variable number tandem repeat analysis (MLVA) trace back investigations frequently identify isolate distribution patterns that may be epidemiologically linked to disease outbreaks. In this study, the in vitro virulence potential of S. Typhimurium DT9 isolates possessing different MLVA patterns (03 15 07 11 550, 03 24 11 10 523, 03 15 08 11 550 and 03 14 08 11 550) isolated from either humans or layer hens was assessed using a human colon carcinoma cell line. Four strains per MLVA from each host for a total of 32 isolates were included in these experiments. Bacteria were grown to stationary phase and added to cells at a multiplicity of infection of 100. Across all isolates, mean percent recovery ranged from 7.1 ± 1.1 to 33.3 ± 7.1%. The layer hen isolate, KC900 (MLVA profile 03 15 08 11 550), exhibited the greatest invasion with a mean percent recovery of 33.3 ± 7.1%. Overall, layer hen isolates of S. Typhimurium DT9 had significantly higher invasion into Caco2 cells than human isolates (p = .0021). RAPD and enterobacterial repetitive intergenic consensus genomic fingerprinting was also performed. 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Four strains per MLVA from each host for a total of 32 isolates were included in these experiments. Bacteria were grown to stationary phase and added to cells at a multiplicity of infection of 100. Across all isolates, mean percent recovery ranged from 7.1 ± 1.1 to 33.3 ± 7.1%. The layer hen isolate, KC900 (MLVA profile 03 15 08 11 550), exhibited the greatest invasion with a mean percent recovery of 33.3 ± 7.1%. Overall, layer hen isolates of S. Typhimurium DT9 had significantly higher invasion into Caco2 cells than human isolates (p = .0021). RAPD and enterobacterial repetitive intergenic consensus genomic fingerprinting was also performed. 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source Wiley-Blackwell Read & Publish Collection
subjects Bacteria
Caco2 cells
Capacity
Cells
Colon
Colorectal cancer
Disease outbreaks
Epidemiology
Fingerprinting
Foodborne diseases
invasion
Microbiological strains
MLVA
Multiplicity of infection
Neoplasms
Outbreaks
Recovery
repetitive element PCR
Salmonella
Salmonella Typhimurium
Salmonellosis
Stationary phase
Virulence
title In vitro invasion capacity of Salmonella Typhimurium DT9 isolates sourced from humans and layer hen environments
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