Single slice method for quantification of hemorrhagic transformation using direct ELISA

An important area of experimental stroke research addresses the development of brain hemorrhage after cerebral ischemia. Investigations of hemorrhagic transformation, however, have been compromised by the absence of sensitive methods for quantification of hemorrhagic transformation. We have develope...

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Bibliographic Details
Published in:Neurological research (New York) 2004-01, Vol.26 (1), p.93-98
Main Authors: Hilali, Hend M., Simpkins, Alexis N., Hill, William D., Waller, Jennifer L., Knight, Robert A., Fagan, Susan C.
Format: Article
Language:English
Subjects:
Animals
Brain Ischemia - complications
Brain Ischemia - pathology
Cerebral Hemorrhage - etiology
Cerebral Hemorrhage - pathology
Cerebral Infarction - etiology
Cerebral Infarction - pathology
Disease Models, Animal
ELISA
Enzyme-Linked Immunosorbent Assay - methods
HEMOGLOBIN
Hemoglobins - analysis
HEMORRHAGIC TRANSFORMATION
In Vitro Techniques
Male
Microtomy - methods
Predictive Value of Tests
RATS
Rats, Wistar
Reproducibility of Results
Sensitivity and Specificity
STROKE
Tetrazolium Salts
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Summary:An important area of experimental stroke research addresses the development of brain hemorrhage after cerebral ischemia. Investigations of hemorrhagic transformation, however, have been compromised by the absence of sensitive methods for quantification of hemorrhagic transformation. We have developed a direct ELISA method that is sensitive, reproducible and can be conducted on tissue treated with triphenyltetrazolium chloride, a stain commonly used for infarct size determination. We have also localized the slices containing the most hemoglobin to facilitate a single slice analysis. This allows two essential variables in experimental stroke research to be assessed on the same slice, leaving the rest of the brain available for other analysis.
ISSN:0161-6412
1743-1328
DOI:10.1179/016164104773026606