Single slice method for quantification of hemorrhagic transformation using direct ELISA

• Published in: Neurological research (New York) 2004-01, Vol.26 (1), p.93-98
• Main Authors: Hilali, Hend M., Simpkins, Alexis N., Hill, William D., Waller, Jennifer L., Knight, Robert A., Fagan, Susan C.
• Format: Article
• Language: English
• Subjects: Animals; Brain Ischemia - complications; Brain Ischemia - pathology; Cerebral Hemorrhage - etiology; Cerebral Hemorrhage - pathology; Cerebral Infarction - etiology; Cerebral Infarction - pathology; Disease Models, Animal; ELISA; Enzyme-Linked Immunosorbent Assay - methods; HEMOGLOBIN; Hemoglobins - analysis; HEMORRHAGIC TRANSFORMATION; In Vitro Techniques; Male; Microtomy - methods; Predictive Value of Tests; RATS; Rats, Wistar; Reproducibility of Results; Sensitivity and Specificity; STROKE; Tetrazolium Salts
• ISSN: 0161-6412; 1743-1328
• DOI: 10.1179/016164104773026606

An important area of experimental stroke research addresses the development of brain hemorrhage after cerebral ischemia. Investigations of hemorrhagic transformation, however, have been compromised by the absence of sensitive methods for quantification of hemorrhagic transformation. We have developed a direct ELISA method that is sensitive, reproducible and can be conducted on tissue treated with triphenyltetrazolium chloride, a stain commonly used for infarct size determination. We have also localized the slices containing the most hemoglobin to facilitate a single slice analysis. This allows two essential variables in experimental stroke research to be assessed on the same slice, leaving the rest of the brain available for other analysis.