Calcium chloride‐enriched calcium aluminate cement promotes in vitro osteogenesis

• Published in: International endodontic journal 2018-06, Vol.51 (6), p.674-683
• Main Authors: Castro‐Raucci, L. M. S., Teixeira, L. N., Barbosa, A. F. S., Fernandes, R. R., Raucci‐Neto, W., Jacobovitz, M., Oliveira, I. R., de Oliveira, P. T.
• Format: Article
• Language: English
• Subjects: Aluminum Compounds - pharmacology; Animals; Apoptosis; Bismuth - pharmacology; bismuth oxide; Bone healing; calcium aluminate cement; Calcium chloride; Calcium Chloride - pharmacology; Calcium Compounds - pharmacology; Calvaria; Cell culture; cell differentiation; Cell Survival; Cells, Cultured; Cement; Culture media; Dental Cements - chemistry; Dental Cements - pharmacology; Dentistry; Endodontics; Genotype & phenotype; Mineralization; Osteoblastogenesis; Osteoblasts; Osteogenesis; Osteogenesis - drug effects; Phenotype; Phenotypes; Phosphorus; Rats; Rats, Wistar; root canal filling materials; Skull - cytology; Zinc oxide; Zinc Oxide - pharmacology; Zinc oxides
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/iej.12883

Aim To evaluate the effects of 2.8% or 10% calcium chloride (CaCl2) in calcium aluminate cement (CAC) with either bismuth oxide (Bi2O3) or zinc oxide (ZnO) as radiopacifiers on the progression of osteogenic cell cultures. Methodology Rat calvaria‐derived cells were grown on Thermanox® coverslips for 24 h and exposed to samples of (i) CACb: with 2.8% CaCl2 and 25% Bi2O3; (ii) CACb+: with 10% CaCl2 and 25% Bi2O3; (iii) CACz: with 2.8% CaCl2 and 25% ZnO; or (iv) CACz+: with 10% CaCl2 and 25% ZnO, placed on inserts. Nonexposed cultures served as the control. Calcium and phosphorus contents in culture media were quantified. The effects of the cements on cell apoptosis, cell viability and acquisition of the osteogenic cell phenotype were evaluated. Data were compared by Kruskal–Wallis test (α = 5%). Results CACb+ promoted the highest levels of calcium in the culture media; CACz+, the lowest levels of phosphorus (P