miR‐24‐3p promotes cell migration and proliferation in lung cancer by targeting SOX7

• Published in: Journal of cellular biochemistry 2018-05, Vol.119 (5), p.3989-3998
• Main Authors: Yan, Liang, Ma, Jinzhu, Zhu, Yiping, Zan, Jiawei, Wang, Zhen, Ling, Liefeng, Li, Qiang, Lv, Jun, Qi, Shimei, Cao, Yingya, Liu, Ying, Cao, Long, Zhang, Yao, Qi, Zhilin, Nie, Liuwang
• Format: Article
• Language: English
• Subjects: A549 Cells; Bioinformatics; Cancer; Cell adhesion & migration; Cell growth; Cell migration; Cell Movement; Cell Proliferation; Chromosome 3; Humans; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; MicroRNAs - genetics; MicroRNAs - metabolism; miR‐24‐3p; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Polymerase chain reaction; RNA, Neoplasm - genetics; RNA, Neoplasm - metabolism; SOX7; Sox7 protein; SOXF Transcription Factors - genetics; SOXF Transcription Factors - metabolism; Transcription; Western blotting; Xenografts; Xenotransplantation
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.26553

Lung cancer (LC) is one of the leading causes of cancer‐related death in the world. miR‐24‐3p plays critical roles in many cancer types, including LC. In this study, we first investigated whether miR‐24‐3p promoted LC cell migration and proliferation in vitro. We used three bioinformatics algorithms to predict the miR‐24‐3p target gene to study the molecular mechanism by which miR‐24‐3p contributes to LC progression. Then, we used the luciferase reporter assay to identify whether SOX7 was a direct target of miR‐24‐3p. Moreover, Western blotting and a quantitative real time‐polymerase chain reaction analysis showed that miR‐24‐3p downregulated SOX7 protein expression by a post‐transcriptional mechanism. Finally, we determined that SOX7 had opposing effects to those of miR‐24‐3p on LC cell proliferation and migration, suggesting that miR‐24‐3p promotes cell proliferation and migration by directly targeting SOX7. Furthermore, miR‐24‐3p accelerated tumor growth in xenograft mice by targeting SOX7. These results provide the first clue that miR‐24‐3p could play a role as an oncomiR in LC by regulating SOX7. Our results provided the first clue that miR‐24‐3p could play an oncomiR role in LC by regulating SOX7. Further studies should consider the relationship between miR‐24‐3p and SOX7, which may reveal a novel strategy for LC treatment.