Re-engineering the two-component systems as light-regulated in Escherichia coli

Bacteria live in environments with dynamic changes. To sense and respond to different external stimuli, bacteria make use of various sensor-response circuits, called two-component systems (TCSs). A TCS comprises a histidine protein kinase (HK) sensing environmental stimuli and a response regulator p...

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Bibliographic Details
Published in:Journal of biosciences 2017-12, Vol.42 (4), p.565-573
Main Authors: Ma, Siya, Luo, Siwei, Wu, Li, Liang, Zhi, Wu, Jia-Rui
Format: Article
Language:English
Subjects:
Bacteria
Biomedical and Life Sciences
Biomedicine
Cell Biology
Chimeras
Cyanobacteria
Detection
E coli
Environmental effects
Escherichia coli
External stimuli
Frequency dependence
Frequency response
Fusion protein
Genes
Genetics
Histidine
Information processing
Kinases
Libraries
Life Sciences
Light
Microbiology
Optics
Phosphorylation
Plant Sciences
Protein kinase
Proteins
Reengineering
Sensors
Stability
Stimuli
Tissue engineering
Zoology
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Summary:Bacteria live in environments with dynamic changes. To sense and respond to different external stimuli, bacteria make use of various sensor-response circuits, called two-component systems (TCSs). A TCS comprises a histidine protein kinase (HK) sensing environmental stimuli and a response regulator protein (RR) regulating downstream genes. The two components are coupled via a phosphorylation control mechanism. In a recent study, we adopted an optogenetics approach to re-engineer the sensor HKs in Escherichia coli as a light-sensing fusion protein. We constructed a light-controllable HK by replacing the original signal-specific sensing domain of HK with the light-sensing domain of Cph1 from Cyanobacteria Synechocystis , so that HK can be investigated by red light. Here, we extended the study to other 16 HK-RR TCSs and constructed a library of light-responsible HK-Cph1 chimeras. By taking the NarX-NarL system as an example, we demonstrated the light responsiveness of the constructed chimera and investigated the frequency response of the NarX-NarL system. The constructed library serves as a toolkit for future TCS study using optogenetics approach.
ISSN:0250-5991
0973-7138
DOI:10.1007/s12038-017-9711-8