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Inhibitory effects of edible marine algae extracts on degranulation of RBL-2H3 cells and mouse eosinophils
Inhibitory effects on degranulation of rat basophilic leukemia (RBL-2H3) cells and mouse eosinophils by marine algae extracts were examined. More than 50% of the degranulation of RBL-2H3 cells was inhibited by water extracts of Ecklonia cava and Chrysymenia wrightii at a concentration of 100 μg/mL....
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Published in: | Fisheries science 2008-10, Vol.74 (5), p.1157-1165 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Inhibitory effects on degranulation of rat basophilic leukemia (RBL-2H3) cells and mouse eosinophils by marine algae extracts were examined. More than 50% of the degranulation of RBL-2H3 cells was inhibited by water extracts of
Ecklonia cava
and
Chrysymenia wrightii
at a concentration of 100 μg/mL. More than 50% of the degranulation of RBL-2H3 cells was inhibited by methanol extracts of
Petalonia binghamiae, Scytosiphon lomentaria, Undaria pinnatifida, Porphyra dentata, Codium fragile
and
Ulva japonica
at a concentration of 200 μg/mL. Most inhibitory substances in the methanol extracts were partitioned into ethyl acetate and hexane layers. The ethyl acetate-partitioned layer of methanol extract of
Petalonia binghamiae
had higher inhibitory effects than the hexane-partitioned layer on the degranulation of RBL-2H3 cells. By contrast, the hexanepartitioned layer of the same extract had a higher inhibitory effect than the ethyl acetate-partitioned layer on the degranulation of mouse eosinophils. The ethyl acetate-partitioned layer of methanol extract of
Petalonia binghamiae
was further separated into eight fractions by silica gel column chromatography. Most inhibited the degranulation of RBL-2H3 cells, but not that of mouse eosinophils significantly. These results suggest methanol extract of
Petalonia binghamiae
contains materials that inhibit the degranulation of basophils and eosinophils differentially. |
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ISSN: | 0919-9268 1444-2906 |
DOI: | 10.1111/j.1444-2906.2008.01635.x |