G Protein Coupling and Second Messenger Generation Are Indispensable for Metalloprotease-dependent, Heparin-binding Epidermal Growth Factor Shedding through Angiotensin II Type-1 Receptor

• Published in: The Journal of biological chemistry 2005-07, Vol.280 (28), p.26592-26599
• Main Authors: Mifune, Mizuo, Ohtsu, Haruhiko, Suzuki, Hiroyuki, Nakashima, Hidekatsu, Brailoiu, Eugen, Dun, Nae J., Frank, Gerald D., Inagami, Tadashi, Higashiyama, Shigeki, Thomas, Walter G., Eckhart, Andrea D., Dempsey, Peter J., Eguchi, Satoru
• Format: Article
• Language: English
• Subjects: ADAM Proteins; ADAM17 Protein; Adenoviridae - genetics; Adenovirus; Alkaline Phosphatase - metabolism; Animals; Calcium - metabolism; CHO Cells; COS Cells; Cricetinae; Epidermal Growth Factor - chemistry; Epidermal Growth Factor - metabolism; Epidermal Growth Factor - physiology; GTP-Binding Proteins - chemistry; GTP-Binding Proteins - metabolism; Heparin - chemistry; Heparin-binding EGF-like Growth Factor; Hydrogen Peroxide - pharmacology; Intercellular Signaling Peptides and Proteins; Ionophores - pharmacology; Kinetics; Lac Operon; Ligands; Metalloendopeptidases - metabolism; Metalloproteases - metabolism; Mutation; Protein Binding; Rats; Reactive Oxygen Species; Receptor, Angiotensin, Type 2 - chemistry; Retroviridae - genetics; Signal Transduction; Time Factors; Transcriptional Activation; Transfection; Type C Phospholipases - metabolism
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M502906200

A G protein-coupled receptor agonist, angiotensin II (AngII), induces epidermal growth factor (EGF) receptor (EGFR) transactivation possibly through metalloprotease-dependent, heparin-binding EGF (HB-EGF) shedding. Here, we have investigated signal transduction of this process by using COS7 cells expressing an AngII receptor, AT1. In these cells AngII-induced EGFR transactivation was completely inhibited by pretreatment with a selective HB-EGF inhibitor, or with a metalloprotease inhibitor. We also developed a COS7 cell line permanently expressing a HB-EGF construct tagged with alkaline phosphatase, which enabled us to measure HB-EGF shedding quantitatively. In the COS7 cell line AngII stimulated release of HB-EGF. This effect was mimicked by treatment either with a phospholipase C activator, a Ca2+ ionophore, a metalloprotease activator, or H2O2. Conversely, pretreatment with an intracellular Ca2+ antagonist or an antioxidant blocked AngII-induced HB-EGF shedding. Moreover, infection of an adenovirus encoding an inhibitor of Gq markedly reduced EGFR transactivation and HB-EGF shedding through AT1. In this regard, AngII-stimulated HB-EGF shedding was abolished in an AT1 mutant that lacks Gq protein coupling. However, in cells expressing AT1 mutants that retain Gq protein coupling, AngII is still able to induce HB-EGF shedding. Finally, the AngII-induced EGFR transactivation was attenuated in COS7 cells overexpressing a catalytically inactive mutant of ADAM17. From these data we conclude that AngII stimulates a metalloprotease ADAM17-dependent HB-EGF shedding through AT1/Gq/phospholipase C-mediated elevation of intracellular Ca2+ and reactive oxygen species production, representing a key mechanism indispensable for EGFR transactivation.