Identification of a SCAR marker associated with Bm, the beet mosaic virus resistance gene, on chromosome 1 of sugar beet

• Published in: Plant breeding 2006-04, Vol.125 (2), p.167-172
• Main Authors: Friesen, T.L, Weiland, J.J, Aasheim, M.L, Hunger, S, Borchardt, D.C, Lewellen, R.T
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; alleles; Aphididae; Beet mosaic virus; Beta vulgaris; Beta vulgaris subsp. vulgaris; Biological and medical sciences; Bm gene; chromosome mapping; disease resistance; Fundamental and applied biological sciences. Psychology; genes; genetic markers; Genetics and breeding of economic plants; molecular sequence data; nucleotide sequences; Pest resistance; plant diseases and disorders; Plant pathogens; random amplified polymorphic DNA technique; RAPD; sequence characterized amplified region; single nucleotide polymorphism; SNP; sugar beet; Varietal selection. Specialized plant breeding, plant breeding aims; virus yellows
• ISSN: 0179-9541; 1439-0523
• DOI: 10.1111/j.1439-0523.2006.01179.x

Beet mosaic virus (BtMV) is an aphid transmitted, viral disease of beet found worldwide. The Bm gene, a resistance gene effective against BtMV, was identified in the sugar beet line 8500 and backcrossed into a C37 background to produce line C719. Three populations were developed from the cross of line C719 with the susceptible line C37 with the intent of developing markers for use in marker-assisted selection. The F₂ progeny of three crosses were scored for resistance. Two of the three populations conformed to a 3 : 1 ratio, indicating a single gene trait. Sequence characterized amplified region (SCAR) markers were developed by using bulked segregant analysis combined with random amplified polymorphic DNA type markers. The markers showed close association to the Bm resistance gene and were effective in all three populations. The A[subscript 1] allele for genetic male sterility also was found to be associated with Bm and the SCAR marker. Development of a single-nucleotide polymorphism marker from the SCAR sequence was used to validate linkage to chromosome 1 using separate mapping populations. This marker will be useful for the introgression of the Bm gene into germplasm.