Real-Time Quantitative Broad-Range PCR Assay for Detection of the 16S rRNA Gene Followed by Sequencing for Species Identification

• Published in: Journal of Clinical Microbiology 2006-08, Vol.44 (8), p.2750-2759
• Main Authors: ZUCOL, Franziska, AMMANN, Roland A, BERGER, Christoph, AEBI, Christoph, ALTWEGG, Martin, NIGGLI, Felix K, NADAL, David
• Format: Article
• Language: English
• Subjects: Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteriology; Biological and medical sciences; Blood - microbiology; DNA Primers; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; DNA, Ribosomal - genetics; Endopeptidase K - metabolism; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Humans; Infectious diseases; Lysostaphin - metabolism; Medical sciences; Microbiology; Muramidase - metabolism; Polymerase Chain Reaction - methods; RNA, Ribosomal, 16S - genetics; Sensitivity and Specificity; Sequence Analysis, DNA
• ISSN: 0095-1137; 1098-660X; 1098-5530
• DOI: 10.1128/JCM.00112-06

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