Exposure to airborne microorganisms, dust and endotoxin during processing of peppermint and chamomile herbs on farms

• Published in: Annals of Agricultural and Environmental Medicine 2005, Vol.12 (2), p.281-288
• Main Authors: Skórska, Czesława, Sitkowska, Jolanta, Krysińska-Traczyk, Ewa, Cholewa, Grazyna, Dutkiewicz, Jacek
• Format: Article
• Language: English
• Subjects: Agricultural Workers' Diseases - microbiology; Agriculture; Air Microbiology; Air Pollutants, Occupational - analysis; Air sampling; Airborne microorganisms; Bacteria; Chamomile - microbiology; Dust; Dust - analysis; Endotoxins; Endotoxins - analysis; Enterobacter agglomerans; Erwinia herbicola; Farmers; Farms; Food Industry; Food Microbiology; Fungi; Fungi - isolation & purification; Gram-negative bacteria; Gram-Negative Bacteria - isolation & purification; Gram-Positive Bacteria - isolation & purification; Herbs; Humans; Matricaria recutita; Mentha piperita; Mentha piperita - microbiology; Microflora; Microorganisms; Occupational Exposure - analysis; Pantoea agglomerans; Plants, Medicinal - microbiology; Poland; Respiratory diseases; Risk Assessment; Rural Population; Samplers; Species composition; Vegetables
• ISSN: 1232-1966; 1898-2263

The aim of this study was to determine the levels of microorganisms, dust and endotoxin in the air during processing of peppermint (Mentha piperita) and chamomile (Matricaria recutita) by herb farmers, and to examine the species composition of airborne microflora. Air samples were collected on glass fibre filters by use of personal samplers on 13 farms owned by herb cultivating farmers, located in Lublin province (eastern Poland). The concentrations of total viable microorganisms (bacteria + fungi) in the farm air during processing of peppermint herb were large, within a range from 895.1-6,015.8 x 10(3) cfu/m(3) (median 1,055.3 x 10(3) cfu/m(3)). During processing of chamomile herb they were much lower and varied within a range from 0.88-295.6 x 10(3) cfu/m(3) (median 27.3 x 10(3) cfu/m(3)). Gram-negative bacteria distinctly prevailed during processing of peppermint leaves, forming 46.4-88.5 % of the total airborne microflora. During processing of chamomile herb, Gram-negative bacteria were dominant at 3 out of 6 sampling sites forming 54.7-75.3 % of total microflora, whereas at the remaining 3 sites the most common were fungi forming 46.2-99.9 % of the total count. The species Pantoea agglomerans (synonyms: Erwinia herbicola, Enterobacter agglomerans ), having strong allergenic and endotoxic properties, distinctly prevailed among Gram-negative isolates. Among fungi, the most common species was Alternaria alternata. The concentrations of airborne dust and endotoxin determined on the examined herb farms were large. The concentrations of airborne dust during peppermint and chamomile processing ranged from 86.7-958.9 mg/m(3), and from 1.1-499.2 mg/m(3), respectively (medians 552.3 mg/m(3) and 12.3 mg/m(3)). The concentrations of airborne endotoxin determined during peppermint and chamomile processing were within a wide range 1.53-208.33 microg/m(3) and 0.005-2604.19 microg/m(3) respectively (medians 57.3 microg/m(3) and 0.96 microg/m(3)). In conclusion, farmers cultivating peppermint are exposed during processing of this herb to large concentrations of airborne microorganisms, dust and endotoxin posing a risk of work-related respiratory disease. The exposure to bioaerosols during processing of chamomile is lower; nevertheless, peak values create a respiratory risk for exposed farmers.