Mapping of Rym16 super(Hb), the second soil-borne virus-resistance gene introgressed from Hordeum bulbosum

Rym16 super(Hb), a gene conferring resistance to soil-borne viruses, was introgressed from Hordeum bulbosum to barley chromosome 2HL. Mechanical inoculation with BaMMV and field tests on a plot contaminated with different viruses demonstrated that Rym16 super(Hb) is effective against all European vi...

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Bibliographic Details
Published in:Theoretical and applied genetics 2006-09, Vol.113 (5), p.867-873
Main Authors: Ruge-Wehling, B, Linz, A, Habekuss, A, Wehling, P
Format: Article
Language:English
Subjects:
Hordeum bulbosum
Hordeum vulgare
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Summary:Rym16 super(Hb), a gene conferring resistance to soil-borne viruses, was introgressed from Hordeum bulbosum to barley chromosome 2HL. Mechanical inoculation with BaMMV and field tests on a plot contaminated with different viruses demonstrated that Rym16 super(Hb) is effective against all European viruses of the soil-borne virus complex (BaMMV, BaYMV-1, -2). Genetic analysis revealed a dominant inheritance of the resistance controlled by Rym16 super(Hb). Using 2HL anchor markers, the size of the introgression was estimated to be about 30 M. In its proximal part, the introgression was characterized by a rearrangement of markers Xbcd266, ABC153 and ABC252, accompanied with pronounced linkage drag by factor 4 in segregating mapping populations. The introgression was found to be associated with a recessive lethality factor, l super(Hb), which was closely linked to the markers mentioned above. Recombination occurring within the introgressed H. bulbosum segment allowed us to separate l super(Hb) from Rym16 super(Hb) and to reduce the size of the introgression to 23 cM or less.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-006-0345-8