Naegleria fowleri immunization modifies lymphocytes and APC of nasal mucosa

• Published in: Parasite immunology 2018-03, Vol.40 (3), p.n/a
• Main Authors: Carrasco‐Yepez, M. M., Campos‐Rodríguez, R., Reséndiz‐Albor, A. A., Peña‐Juárez, C., Contis‐Montes de Oca, A., Arciniega‐Martínez, I. M., Bonilla‐Lemus, P., Rojas‐Hernandez, S.
• Format: Article
• Language: English
• Subjects: Administration, Intranasal; Animals; Antigen-presenting cells; Antigen-Presenting Cells - immunology; Antigens, Protozoan - administration & dosage; Antigens, Protozoan - immunology; B-Lymphocytes - immunology; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; CD45 antigen; CD8 antigen; CD8-Positive T-Lymphocytes - immunology; CD80 antigen; CD86 antigen; cervical lymph nodes; Cholera toxin; Cholera Toxin - administration & dosage; Costimulator; Dendritic cells; Epithelium; Flow cytometry; Immunization; Immunoglobulin A; Lymph nodes; Lymph Nodes - immunology; Lymphocytes; Lymphocytes B; Lymphocytes T; Lymphoid tissue; Lysates; Macrophages; Mice; Mice, Inbred BALB C; Mucosa; Naegleria fowleri; Naegleria fowleri - growth & development; Naegleria fowleri - immunology; Naegleria fowleri - physiology; NALT; Nasal Mucosa - cytology; Nasal Mucosa - immunology; nasal passages; Nose; Spleen; Trophozoites
• ISSN: 0141-9838; 1365-3024
• DOI: 10.1111/pim.12508

Summary We investigated whether intranasal immunization with amoebic lysates plus cholera toxin modified the populations of T and B lymphocytes, macrophages and dendritic cells by flow cytometry from nose‐associated lymphoid tissue (NALT), cervical lymph nodes (CN), nasal passages (NP) and spleen (SP). In all immunized groups, the percentage of CD4 was higher than CD8 cells. CD45 was increased in B cells from mice immunized. We observed IgA antibody‐forming cell (IgA‐AFC) response, mainly in NALT and NP. Macrophages from NP and CN expressed the highest levels of CD80 and CD86 in N. fowleri lysates with either CT or CT alone immunized mice, whereas dendritic cells expressed high levels of CD80 and CD86 in all compartment from immunized mice. These were lower than those expressed by macrophages. Only in SP from CT‐immunized mice, these costimulatory molecules were increased. These results suggest that N. fowleri and CT antigens are taking by APCs, and therefore, protective immunity depends on interactions between APCs and T cells from NP and CN. Consequently, CD4 cells stimulate the differentiation from B lymphocytes to AFC IgA‐positive; antibody that we previously found interacting with trophozoites in the nasal lumen avoiding the N. fowleri attachment to nasal epithelium.