The role of human chorionic gonadotropin in regulation of naïve and memory T cells activity in vitro

• Published in: International immunopharmacology 2018-01, Vol.54, p.33-38
• Main Authors: Zamorina, S.A., Litvinova, L.S., Yurova, K.A., Khaziakhmatova, O.G., Timganova, V.P., Bochkova, M.S., Khramtsov, P.V., Rayev, M.B.
• Format: Article
• Language: English
• Subjects: Alternative Splicing; CD25 antigen; CD28 antigen; CD28 Antigens - genetics; CD28 Antigens - metabolism; CD45RA antigen; Cell Differentiation; Cell growth; Cell Proliferation; Cells, Cultured; Chorionic gonadotropin; Chorionic Gonadotropin - immunology; Chorionic Gonadotropin - metabolism; Costimulatory/activation molecules; Cytokines; Gene expression; Gene Expression Regulation; Genes; Genetics; Gonadotropins; Human chorionic gonadotropin; Humans; Immunologic Memory; Immunological memory; Interleukin-2; Interleukin-2 Receptor alpha Subunit - genetics; Interleukin-2 Receptor alpha Subunit - metabolism; Leukocyte Common Antigens - genetics; Leukocyte Common Antigens - metabolism; Lymphocyte Activation; Lymphocytes; Lymphocytes T; Memory cells; Memory T cells; Molecular chains; Naïve T cells; Pituitary (anterior); Protein Isoforms - genetics; Protein Isoforms - metabolism; Regulators; T-Lymphocytes - immunology
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2017.10.029

The role of human chorionic gonadotropin (hCG) in the regulation of molecular genetics factors determining the functional activity of human naïve and memory T cells in vitro was studied. It was found that hCG (10 and 100IU/ml) inhibited CD28 and CD25 expression on the naïve T cells (CD45RA+) and CD25 expression on the memory T cells (CD45R0+). hCG didn't affect the CD71 proliferation marker expression in total. Nevertheless, hCG reduced the percentage of proliferating memory T cells with simultaneous suppression of CD71 expression on proliferating CD45R0+cells. In parallel, expression of U2af1l4, Gfi1, and hnRNPLL genes, which are Ptprc gene alternative splicing regulators was evaluated. It was established that hCG stimulated the expression of U2af1l4 and hnRNPLL genes, responsible for the assembly of CD45R0 in memory T cells, but reduced the expression of Gfi1 in these cells. In general, hCG promotes the differentiation of memory T cells by increasing of CD45R0 expression, but inhibits proliferation and CD25 expression which reflects their functional activity. The influence of human chorionic gonadotropin on naïve and memory T cells activity in vitro [Display omitted] •Human chorionic gonadotropin (hCG) modulates the activity of human naïve and memory T cells in vitro.•hCG reduces the expression of activation markers and increases production of interleukin-2 by cells studied.•hCG suppresses memory T cell proliferation.•Simultaneously, hCG regulates the expression of genes responsible for CD45 alternative splicing.•The data obtained expand the understanding of hCG role in the immune memory regulation.