Isolation and evaluation of cocktail phages for the control of multidrug-resistant Escherichia coli serotype O104: H4 and E. coli O157: H7 isolates causing diarrhea

Escherichia coli serotype O157: H7 and E. coli O104: H4 are well known foodborne pathogens causing sever enteric illness. Using bacteriophages as biocontrol agents of some foodborne pathogens and multidrug-resistant (MDR) bacteria has a great attention nowadays. This study aims to test the effect of...

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Bibliographic Details
Published in:FEMS microbiology letters 2018-02, Vol.365 (2), p.1
Main Authors: Safwat Mohamed, Doaa, Farouk Ahmed, Eman, Mohamed Mahmoud, Abobakr, Abd El-Baky, Rehab Mahmoud, John, James
Format: Article
Language:English
Subjects:
Bacteriophages
Biological control
Care and treatment
Causes of
Control
Culture techniques
Diarrhea
E coli
Escherichia coli
Food
Food irradiation
Foodborne pathogens
Health aspects
Host range
Microbiology
Morphology
Multidrug resistance
Multidrug resistant organisms
Natural & organic foods
Pathogens
Phages
Preservatives
Pseudomonas aeruginosa
Sewage
Transmission electron microscopy
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Summary:Escherichia coli serotype O157: H7 and E. coli O104: H4 are well known foodborne pathogens causing sever enteric illness. Using bacteriophages as biocontrol agents of some foodborne pathogens and multidrug-resistant (MDR) bacteria has a great attention nowadays. This study aims to test the effect of cocktail phages on the growth of some foodborne pathogens and MDR E. coli. Routine conventional PCR was used to confirm the identification of E. coli isolates. Double-layered culture technique was used to isolate phages from sewage water. Morphology of bacteriophage was described using transmission electron microscopy, and spot test was performed to determine host range of the phage cocktail. Phage cocktail of Siphoviridae and Podoviridae family infecting E. coli O157: H7, E. coli O104: H4 and untypeable E. coli (neither O157 nor O104) has been isolated from sewage water. Phage cocktail showed both lytic and lysogenic activity. Lytic activity was observed against E. coli O157: H7, E. coli O104: H4 isolates, Staphylococcus. aureus ATCC6538 and Pseudomonas aeruginosa ATCC 10145, while the lysogenic activity was observed against the untypeable strain. The tested phage cocktail showed a promising inhibitory action on E. coli O157: H7 and O104: H4, S. aureus ATCC6538 and P. aeruginosa ATCC 10145, suggesting the possibility of its use as a biocontrol tool or as natural food preservatives for many food products.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnx275