Effect of Variety and Plant Growth Regulators in MS Medium on Callus Proliferation from Virus Infected Tomato Plant

The experiment was conducted in the Tissue Culture Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh for micropropagation of tomato plant to evaluate the effect of variety and Plant Growth Regulators (PGRs) in MS medium on callus proliferation from infected...

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Published in:Biotechnology (Faisalābād, Pakistan) Pakistan), 2004-06, Vol.3 (2), p.181-186
Main Authors: Uddin, M F, Islam, SMA, Sultana, N, Shiragi, MHK
Format: Article
Language:English
Subjects:
Lycopersicon esculentum
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Summary:The experiment was conducted in the Tissue Culture Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh for micropropagation of tomato plant to evaluate the effect of variety and Plant Growth Regulators (PGRs) in MS medium on callus proliferation from infected tomato plant. Three tomato varieties namely, Bahar, Binatomato-2 and Binatomato-3 were used as plant materials in the present study. Virus infected young internode segments of tomato plants were cultured on MS medium supplemented with different concentrations and combinations of Plant Growth Regulators (PGRs) for callus proliferation. The combination of 0.5 mg L super(-1) NAA + 2.0 mg L super(-1) BAP in MS medium was found the best for inducing healthy and light yellow callus after 15 days culture and callus turned Into yellow at 30 Days After Inoculation (DAI) in all varieties. Calli were fully virus infected which was confirmed by ELISA test. These calli can be used for the production of virus free tomato plant by shoot derived meristem culture.
ISSN:1682-296X
DOI:10.3923/biotech.2004.181.186