method for assessing arbuscular mycorrhizal fungi group distribution in tree roots by intergenic transcribed sequence variation

We identified five taxonomic groups of arbuscular mycorrhizal fungi (AMF) inside roots of young trees of six species of legumes and six species of non-legumes from a field site in southern Costa Rica using an AMF group-specific PCR assay of the intergenic transcribed sequence and 18S rRNA gene fragm...

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Bibliographic Details
Published in:Plant and soil 2007-01, Vol.290 (1-2), p.259-268
Main Authors: Shepherd, Mervyn, Nguyen, Linh, Jones, Megan E, Nichols, J. Doland, Carpenter, F. Lynn
Format: Article
Language:English
Subjects:
Acaulosporaceae
Agronomy. Soil science and plant productions
AMF diversity
Animal, plant and microbial ecology
Biological and medical sciences
Clay soils
Cloning
Forest soils
Fundamental and applied biological sciences. Psychology
Fungi
Gigasporaceae
Glomales
Glomus
Glomus etunicatum
Glomus mosseae
Legumes
Microbiology
Plant roots
Plant species
Plants
Polymerase chain reaction
Roots
Sequencing
Species
Species diversity
Symbiosis
Tropical soils
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Summary:We identified five taxonomic groups of arbuscular mycorrhizal fungi (AMF) inside roots of young trees of six species of legumes and six species of non-legumes from a field site in southern Costa Rica using an AMF group-specific PCR assay of the intergenic transcribed sequence and 18S rRNA gene fragment. Assay specificity was verified by cloning and sequencing representatives from four of the five AMF groups. We found no difference in overall AMF diversity levels between legumes and non-legumes or between plant species. Some groups of AMF may associate more frequently with legumes than others, as Glomus Group A (Glomus mosseae/intradices group) representatives were detected more frequently in legumes than non-legumes relative to Glomus Group B (Glomus etunicatum/claroideum) representatives.
ISSN:0032-079X
1573-5036
DOI:10.1007/s11104-006-9157-5