Oxalic acid induced cytological alterations differ in beans tolerant or susceptible to white mould

Leaf tissues of two white bean (Phaseolus vulgaris L.) cultivars, one susceptible and one tolerant to white mould [Sclerotinia sclerotiorum (Lib.) de Bary], were treated for different times with oxalic acid. Tissues were prepared by both thin sectioning and freeze-fracture techniques for electron mi...

Full description

Saved in:
Bibliographic Details
Published in:The New phytologist 1989-08, Vol.112 (4), p.519-525
Main Author: Tu, J.C. (Agriculture Canada, Harrow, Ontario, Canada)
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Ascomycota
Bathing
Biological and medical sciences
Cell membranes
CELL STRUCTURE
Chloroplasts
Conductivity
DISEASE RESISTANCE
ESTRUCTURA CELULAR
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Lesions
Mesophyll cells
Organelles
Pathology, epidemiology, host-fungus relationships. Damages, economic importance
PERMEABILIDAD
PERMEABILITE
PERMEABILITY
PHASEOLUS VULGARIS
Phytopathology
Phytopathology. Animal pests. Plant and forest protection
Plasma membrane
PLASMA MEMBRANES
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
SCLEROTINIA
SCLEROTINIA SCLEROTIORUM
STRUCTURE CELLULAIRE
VARIEDADES
VARIETE
VARIETIES
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Leaf tissues of two white bean (Phaseolus vulgaris L.) cultivars, one susceptible and one tolerant to white mould [Sclerotinia sclerotiorum (Lib.) de Bary], were treated for different times with oxalic acid. Tissues were prepared by both thin sectioning and freeze-fracture techniques for electron microscopy. The plasma membrane of the resistant cultivar appeared more tolerant to damage induced by oxalic acid than that of the susceptible cultivar. At the same concentration of oxalic acid the plasma membrane and chloroplasts of the susceptible cultivar were affected more, and ruptured more quickly, than those of the tolerant one. In thin sections, the ruptured organelles were partially or completely disorganized. In replicas of freeze-fractured preparations of the plasma membrane, increased protrusions, wrinkles, breakages and ruptures were associated with increasing exposure to oxalic acid. Damage was more severe in the plasma membrane of the susceptible cultivar than in that of the tolerant one. Measurements of the conductivity of water bathing leaf discs of the two cultivars showed that the susceptible cultivar led to a higher conductivity than the resistant one, indicating that the injury and rupturing of the plasma membrane were closely related to membrane stability.
ISSN:0028-646X
1469-8137
DOI:10.1111/j.1469-8137.1989.tb00346.x