Isolation and characterization of a mesophilic heavy-metals-tolerant sulfate-reducing bacterium Desulfomicrobium sp. from an enrichment culture using phosphogypsum as a sulfate source

A sulfate-reducing bacterium, was isolated from a 6 month trained enrichment culture in an anaerobic media containing phosphogypsum as a sulfate source, and, designated strain SA2. Cells of strain SA2 were rod-shaped, did not form spores and stained Gram-negative. Phylogenetic analysis of the 16S rR...

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Bibliographic Details
Published in:Journal of hazardous materials 2007-02, Vol.140 (1), p.264-270
Main Authors: Azabou, Samia, Mechichi, Tahar, Patel, Bharat K.C., Sayadi, Sami
Format: Article
Language:English
Subjects:
Applied sciences
Atmospheric pollution
Bacteria
Calcium Sulfate - metabolism
Desulfomicrobium
Exact sciences and technology
Gram-Negative Bacteria
Heavy-metal tolerance
Metals, Heavy - toxicity
Phosphogypsum
Phosphorus - metabolism
Phylogeny
Pollution
Radioactive wastes
Sulfate reduction
Sulfate-reducing bacteria
Sulfur-Reducing Bacteria - cytology
Sulfur-Reducing Bacteria - isolation & purification
Sulfur-Reducing Bacteria - metabolism
Wastes
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Summary:A sulfate-reducing bacterium, was isolated from a 6 month trained enrichment culture in an anaerobic media containing phosphogypsum as a sulfate source, and, designated strain SA2. Cells of strain SA2 were rod-shaped, did not form spores and stained Gram-negative. Phylogenetic analysis of the 16S rRNA gene sequence of the isolate revealed that it was related to members of the genus Desulfomicrobium (average sequence similarity of 98%) with Desulfomicrobium baculatum being the most closely related (sequence similarity of 99%). Strain SA2 used thiosulfate, sulfate, sulfite and elemental sulfur as electron acceptors and produced sulfide. Strain SA2 reduced sulfate contained in 1–20 g/L phosphogypsum to sulfide with reduction of sulfate contained in 2 g/L phosphogypsum being the optimum concentration. Strain SA2 grew with metalloid, halogenated and non-metal ions present in phosphogypsum and with added high concentrations of heavy metals (125 ppm Zn and 100 ppm Ni, W, Li and Al). The relative order for the inhibitory metal concentrations, based on the IC 50 values, was Cu, Te > Cd > Fe, Co, Mn > F, Se > Ni, Al, Li > Zn.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2006.07.073