The use of human cell line reporter gene-based assays in chemical toxicity testing

Genetically modified rodents allow greater sensitivity in monitoring DNA damage or gene expression than traditional rodent bioassays and have become increasingly used for toxicity testing, particularly with the greater availability of protein and DNA-based toxicity biomarkers. Here, the advantages a...

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Bibliographic Details
Published in:Toxicology in vitro 2007-10, Vol.21 (7), p.1233-1240
Main Authors: Scrivens, Michelle, Bhogal, Nirmala
Format: Article
Language:English
Subjects:
Animal Testing Alternatives - methods
Animal welfare
Animals
Big Blue
Carcinogenicity
Carcinogenicity Tests - methods
Cell Line, Tumor
DNA Damage - drug effects
Drug Evaluation, Preclinical - methods
Endocrine
Endocrine Disruptors
Gene Expression Regulation - drug effects
Genes, Reporter - drug effects
Humans
Inflammation - chemically induced
Inflammation - physiopathology
Models, Animal
Mutagenicity
Mutagenicity Tests - methods
Muta™ mouse
Reporter genes
Rodentia
Three Rs
Toxicity
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Summary:Genetically modified rodents allow greater sensitivity in monitoring DNA damage or gene expression than traditional rodent bioassays and have become increasingly used for toxicity testing, particularly with the greater availability of protein and DNA-based toxicity biomarkers. Here, the advantages and limitations of several in vitro reporter assays already used to study the mechanisms of toxicity are discussed in relation to the in vivo traditional and reporter-based bioassays for carcinogenicity, mutagenicity, endocrine changes and inflammation endpoints to examine the scope for refining and replacing transgenic in vivo models.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2007.05.006