Dereplication‐guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time‐of‐flight MS

Uncaria rhynchophylla (Gou‐Teng) as the monarch herb of many formulae (Fufang), e.g. “Tian‐Ma‐Gou‐Teng‐Yin,” “Ling‐Jiao‐Gou‐Teng‐Yin,” and “Yi‐Gan‐San”, is a famous traditional Chinese medicine documented in the Chinese pharmacopoeia for mental and cardiovascular diseases. In the traditional Chinese...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2018-04, Vol.41 (7), p.1532-1538
Main Authors: Zhang, Jian‐Gang, Huang, Xiao‐Yan, Ma, Yun‐Bao, Zhang, Xue‐Mei, Chen, Ji‐Jun, Geng, Chang‐An
Format: Article
Language:English
Subjects:
5‐hydroxytryptamine receptors
Acupuncture
Chinese medicine
Chromatography
Column chromatography
Combinatorial analysis
Constituents
dereplication‐guided isolation
Heart diseases
Herbs
Hooks
indole alkaloid triglycoside
Ions
Mass spectrometry
Medicine
Melatonin
melatonin receptors
Pharmacology
Receptors
Scientific imaging
Spectroscopy
Stems
Traditional Chinese medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Uncaria rhynchophylla (Gou‐Teng) as the monarch herb of many formulae (Fufang), e.g. “Tian‐Ma‐Gou‐Teng‐Yin,” “Ling‐Jiao‐Gou‐Teng‐Yin,” and “Yi‐Gan‐San”, is a famous traditional Chinese medicine documented in the Chinese pharmacopoeia for mental and cardiovascular diseases. In the traditional Chinese medicine system, only the hook‐bearing stems are used as the crude materials for Gou‐Teng, and the hooks are always considered more effective than the stems. Focusing on the mono‐herb and its active constituents from combinatorial formulae is the core idea of reductionism of traditional Chinese medicine theory. Detailed liquid chromatography with mass spectrometry analysis on the hooks of U. rhynchophylla was performed to profile the chemical constituents based on tandem mass spectrometry fragmentation and UV absorption. Under the guidance of liquid chromatography with ion trap/time‐of‐flight mass spectrometry, one new indole alkaloid triglycoside (1), together with five known compounds 2–6 as the main constituents, were isolated from the hooks of U. rhynchophylla by various column chromatography methods. Compound 1 showed moderate activity on MT1 and MT2 melatonin receptors with agonistic rates of 79.6 and 46.3% at the concentration of 1 mM. This dereplication strategy can be equally applicable to rapidly disclose the active constituents of other Chinese herbs through targeted purification.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201701175