Cross-resistance Patterns to Insecticides of Several Chemical Classes Among Listronotus maculicollis (Coleoptera: Curculionidae) Populations With Different Levels of Resistance to Pyrethroids

• Published in: Journal of economic entomology 2018-02, Vol.111 (1), p.391-398
• Main Authors: Kostromytska, Olga S, Wu, Shaohui, Koppenhöfer, Albrecht M
• Format: Article
• Language: English
• Subjects: Animals; Assaying; baseline susceptibility; Chlorpyrifos; Coleoptera; Connecticut; Cross-resistance; Curculionidae; Cyhalothrin; Female; Greenhouses; High resistance; Insecticide Resistance; INSECTICIDE RESISTANCE AND RESISTANCE MANAGEMENT; Insecticides; Insecticides - pharmacology; Insects; Laboratories; Listronotus maculicollis; Male; Metropolitan areas; Mid-Atlantic Region; Pesticides; Pests; Pyrethrins - pharmacology; pyrethroid resistance; Pyrethroids; Spinosad; Toxicity; Weevils - drug effects; Weevils - physiology
• ISSN: 0022-0493; 1938-291X
• DOI: 10.1093/jee/tox345

The annual bluegrass weevil (ABW), ListronotusmaculicollisKirby (Coleoptera: Curculionidae), is the most damaging golf course insect pest in eastern North America. Heavy reliance on synthetic insecticides against this pest has led to widespread problems in controlling ABW with pyrethroid resistance already reported from populations in southern New England.This study evaluated the degree and scope of ABW resistance, determined existing cross-resistance patterns, and confirmed laboratory findings under greenhouse conditions.The susceptibility of 10 ABW populations to insecticides of different chemical classes was assessed in topical, feeding, and greenhouse assays. The level of susceptibility to pyrethroids varied significantly among populations (LD50s ranging 2.4–819.1 ng per insect for bifenthrin and 1.1–362.7 ng for λ-cyhalothrin in the topical assay). Three populations were relatively susceptible to pyrethroids, and seven populations had moderate to high resistance levels (RR50 for bifenthrin ranging 30.5–343.1).The toxicity of chlorpyrifos (RR50s ranging 3.3–15.3), spinosad (RR50s 2.4–7.7), clothianidin (RR50s 4.2–9.7), and indoxacarb (RR50s 2.8–9.7) was decreased for the pyrethroid-resistant populations. Toxicity data for bifenthrin and chlorpyrifos obtained under more realistic greenhouse conditions confirmed laboratory observations, indicating that the topical assay is an accurate method of detection and measurement of resistance level. The current study expanded the previously known geographic range of ABW pyrethroid resistance to include the New York metropolitan area, New Jersey, and eastern Pennsylvania and provided clear evidence of cross-resistance not only within the pyrethroid class but also to several other chemical classes.