A rapid and sensitive method for detecting foodborne pathogens by capillary electrophoresis-based single-strand conformation polymorphism

A parallel analysis method using capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) combined with 16S rRNA gene-specific PCR was developed for rapid and effective detection of five pathogens associated with food poisoning. Conventional detection and identification meth...

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Bibliographic Details
Published in:Food control 2008-11, Vol.19 (11), p.1100-1104
Main Authors: Oh, Mi-Hwa, Park, Young Seoub, Paek, Se-Hee, Kim, Hae-Yeong, Jung, Gyoo Yeol, Oh, Sangsuk
Format: Article
Language:English
Subjects:
16S rRNA gene
Bacillus cereus
Campylobacter jejuni
capillary electrophoresis
Clostridium perfringens
detection
Escherichia coli
food analysis
food pathogens
Foodborne pathogens
genome
polymerase chain reaction
rapid methods
Salmonella enterica
single-stranded conformational polymorphism
SSCP
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Summary:A parallel analysis method using capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) combined with 16S rRNA gene-specific PCR was developed for rapid and effective detection of five pathogens associated with food poisoning. Conventional detection and identification methods for these pathogens are time consuming and labor intensive due to the necessity of separate cultivation of each target strain. Using the developed CE-SSCP method, Escherichia coli, Clostridium perfringens, Campylobacter jejuni, Salmonella enterica, and Bacillus cereus were simultaneously identified with individual corresponding peaks from a mixture of their genomic DNA. This method can be used for rapid investigation of causative agents of food poisoning. Its simplicity and high sensitivity may lead to improved management of food safety.
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2007.11.009