Pectenotoxin-2 abolishes constitutively activated NF-[kappa]B, leading to suppression of NF-[kappa]B related gene products and potentiation of apoptosis

Although pectenotoxin-2 (PTX-2) is known to modify the actin cytoskeleton, very little is known about its apoptosis mechanism. In this study, we investigated whether PTX-2 induces apoptotic effects through suppression of the NF-κB signaling pathway in several leukemia cell types. PTX-2 significantly...

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Bibliographic Details
Published in:Cancer letters 2008-11, Vol.271 (1), p.25-33
Main Authors: Kim, Mun-Ock, Moon, Dong-Oh, Heo, Moon-Soo, Lee, Jae-Dong, Jung, Jee Hyung, Kim, Se-Kwon, Choi, Yung Hyun, Kim, Gi-Young
Format: Article
Language:English
Subjects:
Apoptosis
Cancer
Cell growth
Cytotoxicity
Deoxyribonucleic acid
DNA
Gene expression
Kinases
Leukemia
Phosphorylation
Proteins
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Summary:Although pectenotoxin-2 (PTX-2) is known to modify the actin cytoskeleton, very little is known about its apoptosis mechanism. In this study, we investigated whether PTX-2 induces apoptotic effects through suppression of the NF-κB signaling pathway in several leukemia cell types. PTX-2 significantly induced growth inhibition and apoptosis in a dose-dependent manner. Treatment with PTX-2 also significantly increased caspase-3 activity and poly (ADP-ribose) polymerase (PARP) cleavage, however caspase-3 inhibitor z-DEVD-fmk significantly inhibited PTX-2-induced cell death. These data suggest that the activation of caspase-3 is associated with PTX-2-induced apoptosis. NF-κB has also been shown to inhibit apoptosis in response to chemotherapeutic agents. As examined by the DNA-binding of NF-κB activation, we found that PTX-2 suppressed constitutive NF-κB activation and determined by p65 and p50 nuclear translocation, and IκBα degradation through dephosphorylation of Akt. Attenuation of constitutive NF-κB activity by pretreatment with pyrrolidine dithiocarbamate (PDTC), an NF-κB nuclear translocation inhibitor, induced significantly apoptosis in the presence of PTX-2. In addition, treatment of PTX-2 down-regulated NF-κB-dependent gene expression, Cox-2, IAP-1, IAP-2 and XIAP, at the transcriptional and translational level. Taken together, these results suggest that anti-cancer activities induced by PTX-2 may be mediated in part through suppression of constitutive NF-κB activity.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2008.05.034