Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins ( Strongylocentrotus droebachiensis) and mussels ( Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow syste...

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Bibliographic Details
Published in:Marine environmental research 2004-08, Vol.58 (2), p.701-705
Main Authors: Taban, Ingrid Christina, Bechmann, Renée Katrin, Torgrimsen, Sigfryd, Baussant, Thierry, Sanni, Steinar
Format: Article
Language:English
Subjects:
Analysis of Variance
Animal and plant ecology
Animal, plant and microbial ecology
Animals
Assays
Biological and medical sciences
Bivalvia - genetics
Comet Assay
DNA Damage - drug effects
DNA Damage - genetics
DNA strand breaks
Dose-Response Relationship, Drug
Echinoidea
Environmental Monitoring - methods
Fundamental and applied biological sciences. Psychology
Genotoxicants
Hemocytes - metabolism
Marine
Mussel
Mytilus edulis
Oil
Petroleum - toxicity
Sea urchin
Sea Urchins - genetics
Sea water ecosystems
Strongylocentrotus droebachiensis
Synecology
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Summary:The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins ( Strongylocentrotus droebachiensis) and mussels ( Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.
ISSN:0141-1136
1879-0291
DOI:10.1016/j.marenvres.2004.03.018