Phytotoxicity of the tetramic acid metabolite trichosetin

The first details of the phytotoxicity of trichosetin are reported. Trichosetin, a tetramic acid-containing metabolite produced in the dual culture of Trichoderma harzianum and Catharanthus roseus (L.) G. Don callus, was subjected to phytotoxicity assays. In seedling growth assays, trichosetin inhib...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 2003-03, Vol.62 (5), p.715-721
Main Authors: Marfori, Eufrocinio C, Kajiyama, Shin' ichiro, Fukusaki, Ei-ichiro, Kobayashi, Akio
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Catharanthus roseus
Cell Survival - drug effects
Chemical agents
Dose-Response Relationship, Drug
Dual culture
Economic plant physiology
Electrolytes - analysis
Fundamental and applied biological sciences. Psychology
Growth and development
Lipid Peroxidation - drug effects
Magnoliopsida - cytology
Magnoliopsida - drug effects
Magnoliopsida - growth & development
Malondialdehyde - analysis
Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence
Nicotiana tabacum
Phytotoxicity
Plant Growth Regulators - pharmacology
Plant physiology and development
Plant Structures - cytology
Plant Structures - drug effects
Plant Structures - growth & development
Plants, Medicinal - chemistry
Pyrrolidinones - chemistry
Pyrrolidinones - toxicity
Rhodamines
Species Specificity
Tenuazonic Acid - pharmacology
Tetramic acid
Time Factors
Trichosetin
Vegetative apparatus, growth and morphogenesis. Senescence
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Summary:The first details of the phytotoxicity of trichosetin are reported. Trichosetin, a tetramic acid-containing metabolite produced in the dual culture of Trichoderma harzianum and Catharanthus roseus (L.) G. Don callus, was subjected to phytotoxicity assays. In seedling growth assays, trichosetin inhibited root and shoot growth of all five plant species tested by damaging the cell membrane, as evidenced by the dose-dependent increase in electrolyte leakage and lipid peroxidation. Vital staining of trichosetin-treated Nicotiana tabacum BY-2 cells, with rhodamine 123, showed a weaker green fluorescence compared to controls indicating damaging effects on mitochondria. FDA-PI staining, to determine cell viability, indicated that cells of the trichosetin-treated roots were mostly dead.
ISSN:0031-9422
1873-3700
DOI:10.1016/S0031-9422(02)00629-5