Isolation and RNA silencing of homologues of the RNase L inhibitor in Nicotiana species

Based on the sequences of mammalian RNase L inhibitors (RLI), homologues were identified in Arabidopsis thaliana and Nicotiana plant species. RLI homologue (RLIh) cDNAs were cloned from Nicotiana benthamiana and Nicotiana tabacum and used to induce post-transcriptional gene silencing (PTGS) in Nicot...

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Bibliographic Details
Published in:Plant science (Limerick) 2004-12, Vol.167 (6), p.1283-1289
Main Authors: Petersen, Birgit Otzen, Jørgensen, Bodil, Albrechtsen, Merete
Format: Article
Language:English
Subjects:
Animal and plant ecology
Animal, plant and microbial ecology
Arabidopsis thaliana
Autoecology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Nicotiana
Nicotiana benthamiana
Nicotiana tabacum
Plants and fungi
Potato virus Y
RNase L inhibitor
Virus-induced gene silencing
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Summary:Based on the sequences of mammalian RNase L inhibitors (RLI), homologues were identified in Arabidopsis thaliana and Nicotiana plant species. RLI homologue (RLIh) cDNAs were cloned from Nicotiana benthamiana and Nicotiana tabacum and used to induce post-transcriptional gene silencing (PTGS) in Nicotiana species. Downregulation of RLIh in N. benthamiana plants by virus-induced gene silencing had severe effects on plant morphology and plant growth but did not affect the susceptibility of the plant to infection by Potato virus Y (PVY). Upon transformation of N. benthamiana and N. tabacum with an inverted repeat of RLIh only one transformant, X#2.1, displayed an abnormal phenotype characterized by reduced growth and distorted leaves with white spots. Northern blotting revealed the presence of RLIh-specific small interfering RNAs in X#2.1 but not in 14 other transformants with a normal phenotype. Based on our observations we propose that RLIh has an essential role in plant growth and development.
ISSN:0168-9452
1873-2259
DOI:10.1016/j.plantsci.2004.06.030