Conserved genomes of ΦKMV-like bacteriophages (T7 supergroup) active on Pseudomonas aeruginosa

The T7-like Phi KMV bacteriophage active on Pseudomonas aeruginosa was previously isolated by us and shown to have DNA resistant to many endonucleases. A loss of sensitive sites might be a consequence of a long Phi KMV evolution on different hosts. To elucidate, whether this trait is shared by other...

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Bibliographic Details
Published in:Russian journal of genetics 2006-01, Vol.42 (1), p.27-31
Main Authors: Burkaltseva, M. V., Pleteneva, E. A., Shaburova, O. V., Kadykov, V. A., Krylov, V. N.
Format: Article
Language:English
Subjects:
Bacteria
Pseudomonas aeruginosa
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Summary:The T7-like Phi KMV bacteriophage active on Pseudomonas aeruginosa was previously isolated by us and shown to have DNA resistant to many endonucleases. A loss of sensitive sites might be a consequence of a long Phi KMV evolution on different hosts. To elucidate, whether this trait is shared by other similar phages, several new Phi KMV-like phages were isolated from different sources and compared. All studied Phi KMV-like phages formed three groups, insignificantly differing in the number and localization of endonuclease-sensitive DNA sites. This confirms that the present-day phages of this species have highly conserved genomes. Mutational 'restoration' of the lost sites may be restricted by a lethal effect. The Phi KMV-like phages were shown for the first time to increase the rate of in vitro accumulation of giant KZ-like phages of P. aeruginosa. This effect is characteristic only of Phi KMV-like phages.
ISSN:1022-7954
1608-3369
DOI:10.1134/S1022795406010030