Trillium tschonoskii maxim saponin mitigates D-galactose-induced brain aging of rats through rescuing dysfunctional autophagy mediated by Rheb-mTOR signal pathway

• Published in: Biomedicine & pharmacotherapy 2018-02, Vol.98, p.516-522
• Main Authors: Wang, Lingjie, Du, Junlong, Zhao, Fangyu, Chen, Zonghai, Chang, Jingru, Qin, Furong, Wang, Zili, Wang, Fengjie, Chen, Xianbing, Chen, Ning
• Format: Article
• Language: English
• Subjects: Aging - drug effects; Aging - metabolism; Animals; Autophagy; Autophagy - drug effects; Brain aging; China; Cognitive capacity; Galactose - pharmacology; Hippocampus - drug effects; Hippocampus - metabolism; Maxim (TTM); Memory - drug effects; Plants, Medicinal - chemistry; Ras Homolog Enriched in Brain Protein - metabolism; Rats; Rats, Sprague-Dawley; Rheb-mTOR signal pathway; Saponins - pharmacology; Signal Transduction - drug effects; TOR Serine-Threonine Kinases - metabolism; Trillium - chemistry; Trillium tschonoskii
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2017.12.046

•Impaired cognitive capacity is correlated with dysfunctional autophagy regulated by Rheb-mTOR signal pathway.•TTM can up-regulate Rheb and down-regulate mTOR.•TTM can rescue dysfunctional autophagy.•TTM can improve cognitive capacity and mitigate brain-aging process. During the expansion of aging population, the study correlated with brain aging is one of the important research topics. Developing novel and effective strategies for delaying brain aging is highly desired. Brain aging is characteristics of impaired cognitive capacity due to dysfunctional autophagy regulated by Rheb-mTOR signal pathway in hippocampal tissues. In the present study, we have established a rat model with brain aging through subcutaneous injection of D-galactose (D-gal). Upon the intervention of Trillium tschonoskii Maxim (TTM) saponin, one of bioactive components from local natural herbs in China, the learning and memory capacity of D-gal-induced aging rats was evaluated through Morris water maze test, and the regulation of Rheb-mTOR signal pathway and functional status of autophagy in hippocampal tissues of D-gal-induced aging rats was explored by Western blot. TTM saponin revealed an obvious function to improve learning and memory capacity of D-gal-induced aging rats through up-regulating Rheb and down-regulating mTOR, thereby rescuing dysfunctional autophagy to execute anti-aging role. Meanwhile, this study confirmed the function of TTM saponin for preventing and treating brain aging, and provided a reference for the development and utilization of natural products in health promotion and aging-associated disease treatment.