Image analysis for quantification of bacterial rock weathering

A fast, quantitative image analysis technique was developed to assess potential rock weathering by bacteria. The technique is based on reduction in the surface area of rock particles and counting the relative increase in the number of small particles in ground rock slurries. This was done by recordi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microbiological methods 2006-02, Vol.64 (2), p.275-286
Main Authors: Puente, M. Esther, Rodriguez-Jaramillo, M. Carmen, Li, Ching Y., Bashan, Yoav
Format: Article
Language:English
Subjects:
Agriculture
Architecture
Azospirillum
Azospirillum brasilense
Azospirillum brasilense - metabolism
Bacteria
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biodegradation, Environmental
Biological and medical sciences
Electronics
Fundamental and applied biological sciences. Psychology
Genetics
Geologic Sediments - analysis
Image analysis
Image Processing, Computer-Assisted - methods
Microbiology
Microscopy, Phase-Contrast
Pseudomonas
Pseudomonas putida
Pseudomonas putida - metabolism
Rock weathering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A fast, quantitative image analysis technique was developed to assess potential rock weathering by bacteria. The technique is based on reduction in the surface area of rock particles and counting the relative increase in the number of small particles in ground rock slurries. This was done by recording changes in ground rock samples with an electronic image analyzing process. The slurries were previously amended with three carbon sources, ground to a uniform particle size and incubated with rock weathering bacteria for 28 days. The technique was developed and tested, using two rock-weathering bacteria Pseudomonas putida R-20 and Azospirillum brasilense Cd on marble, granite, apatite, quartz, limestone, and volcanic rock as substrates. The image analyzer processed large number of particles (10 7–10 8 per sample), so that the weathering capacity of bacteria can be detected.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2005.05.007