Image analysis for quantification of bacterial rock weathering

• Published in: Journal of microbiological methods 2006-02, Vol.64 (2), p.275-286
• Main Authors: Puente, M. Esther, Rodriguez-Jaramillo, M. Carmen, Li, Ching Y., Bashan, Yoav
• Format: Article
• Language: English
• Subjects: Agriculture; Architecture; Azospirillum; Azospirillum brasilense; Azospirillum brasilense - metabolism; Bacteria; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biodegradation, Environmental; Biological and medical sciences; Electronics; Fundamental and applied biological sciences. Psychology; Genetics; Geologic Sediments - analysis; Image analysis; Image Processing, Computer-Assisted - methods; Microbiology; Microscopy, Phase-Contrast; Pseudomonas; Pseudomonas putida; Pseudomonas putida - metabolism; Rock weathering
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2005.05.007

A fast, quantitative image analysis technique was developed to assess potential rock weathering by bacteria. The technique is based on reduction in the surface area of rock particles and counting the relative increase in the number of small particles in ground rock slurries. This was done by recording changes in ground rock samples with an electronic image analyzing process. The slurries were previously amended with three carbon sources, ground to a uniform particle size and incubated with rock weathering bacteria for 28 days. The technique was developed and tested, using two rock-weathering bacteria Pseudomonas putida R-20 and Azospirillum brasilense Cd on marble, granite, apatite, quartz, limestone, and volcanic rock as substrates. The image analyzer processed large number of particles (10 7–10 8 per sample), so that the weathering capacity of bacteria can be detected.