Symptom development and fungal colonization in tomato lines interacting with Crinipellis perniciosa, the causal agent of cacao witches' broom

We have developed a pathogenicity assay on tomato with strains of the S-biotype of Crinipellis perniciosa (CP). Artificial inoculation on 3-week-old tomato seedlings was performed with a basidiospore suspension at 6 x 10 super(5) concentration. The symptoms observed (swelling, axillary bud activatio...

Full description

Saved in:
Bibliographic Details
Published in:Phytopathology 2005-06, Vol.95 (6)
Main Authors: Marelli, J-P, Rang, S, Maximova, S, Gramacho, K P, Backman, P, Guiltinan, MJ
Format: Article
Language:English
Subjects:
Crinipellis perniciosa
Lycopersicon esculentum
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed a pathogenicity assay on tomato with strains of the S-biotype of Crinipellis perniciosa (CP). Artificial inoculation on 3-week-old tomato seedlings was performed with a basidiospore suspension at 6 x 10 super(5) concentration. The symptoms observed (swelling, axillary bud activation, and elongation) varied considerably between inoculated plants. To investigate the causes of this variability, we followed the locations of CP colonization within infected plants. This was achieved by plating excised tomato tissue from different plant parts on selective media, amplifying CP DNA with specific PCR primers, and microscopically observing stained cross sections of infected tomato tissue. These approaches will advance our knowledge on the infection process of CP in tomato and will aid in the development of a model for understanding disease mechanisms in cacao trees.
ISSN:0031-949X